New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories

• Published in: Journal of biomolecular screening Vol. 21; no. 3; pp. 298 - 305
• Main Authors: Tewson, Paul H., Martinka, Scott, Shaner, Nathan C., Hughes, Thomas E., Quinn, Anne Marie
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.03.2016
• Subjects: Automation, Laboratory; Baculoviridae - physiology; Biosensing Techniques; Cyclic AMP - chemistry; Cyclic AMP - metabolism; Diglycerides - chemistry; Diglycerides - metabolism; HEK293 Cells; Humans; Reproducibility of Results; Transduction, Genetic; GFP; diacyl glycerol; baculovirus; fluorescence; adenylyl cyclase; live-cell assay; DAG; mNeonGreen; cAMP; Z′ statistic

Protein-based, fluorescent biosensors power basic research on cell signaling in health and disease, but their use in automated laboratories is limited. We have now created two live-cell assays, one for diacyl glycerol and another for cAMP, that are robust (Z′ > 0.7) and easily deployed on standar...