Polo-like kinase-1 as a novel target in neoplastic mast cells: demonstration of growth-inhibitory effects of small interfering RNA and the Polo-like kinase-1 targeting drug BI 2536

• Published in: Haematologica (Roma) Vol. 96; no. 5; pp. 672 - 680
• Main Authors: Peter, B., Gleixner, K. V., Cerny-Reiterer, S., Herrmann, H., Winter, V., Hadzijusufovic, E., Ferenc, V., Schuch, K., Mirkina, I., Horny, H.-P., Pickl, W. F., Mullauer, L., Willmann, M., Valent, P.
• Format: Journal Article
• Language: English
• Published: Pavia Ferrata Storti Foundation 01.05.2011
• Subjects: Animals; Apoptosis - drug effects; Biological and medical sciences; Cell Cycle - drug effects; Cell Cycle Proteins - antagonists & inhibitors; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; Dose-Response Relationship, Drug; Flow Cytometry; Hematologic and hematopoietic diseases; HL-60 Cells; Humans; Immunohistochemistry; K562 Cells; Mast Cells - drug effects; Mast Cells - metabolism; Mast Cells - pathology; Mastocytosis, Systemic - genetics; Mastocytosis, Systemic - metabolism; Mastocytosis, Systemic - pathology; Medical sciences; Original; Phosphorylation; Polo-Like Kinase 1; Protein Serine-Threonine Kinases - antagonists & inhibitors; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Proto-Oncogene Proteins - antagonists & inhibitors; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Pteridines - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; Thymidine - metabolism; Thymidine - pharmacokinetics; Tumor Cells, Cultured; U937 Cells; Cell proliferation; Mastocytosis; RNA interference; Targeting; Hematology; Plk-1; siRNA; Malignant tumor; Gene silencing; Cell death; C-Onc gene; Targeted drug; KIT; Mast cell; Target cell; targeted drugs; Protooncogene; Cancer; Apoptosis
• ISSN: 0390-6078; 1592-8721; 1592-8721
• DOI: 10.3324/haematol.2010.031328

In advanced systemic mastocytosis the response of neoplastic mast cells to conventional drugs is poor and the prognosis is bad. Current research is, therefore, attempting to identify novel drug targets in neoplastic mast cells. Polo-like kinase-1 is a serine/threonine kinase that plays an essential...