Significance of indeterminate third-generation hepatitis C virus recombinant immunoblot assay

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Published inJournal of Clinical Microbiology Vol. 34; no. 1; pp. 80 - 83
Main Authors PAWLOTSKY, J.-M, BASTIE, A, PELLET, C, REMIRE, J, DARTHUY, F, WOLFE, L, SAYADA, C, DUVAL, J, DHUMEAUX, D
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.01.1996
Subjects
AIDS/HIV
Antigens, Viral - genetics
Biological and medical sciences
Diagnostic Errors
Evaluation Studies as Topic
Genotype
Hepacivirus - genetics
Hepacivirus - immunology
Hepatitis C - diagnosis
Hepatitis C - immunology
Hepatitis C - virology
Hepatitis C Antibodies - blood
hepatitis C virus
Human viral diseases
Humans
Immune Tolerance
Immunoblotting - methods
Immunocompetence
Infectious diseases
Medical sciences
Polymerase Chain Reaction
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Risk Factors
RNA, Viral - genetics
RNA, Viral - isolation & purification
Viral diseases
Viral hepatitis
Virus Replication
Human
Antibody
Hepatic disease
Method
Infection
Virus
Viral disease
Serological method
Digestive diseases
Diagnosis
Flaviviridae
Detection
Hepatitis C virus
Test interpretation
Viral hepatitis C
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Abstract Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
AbstractList Indeterminate hepatitis C virus (HCV) third-generation recombinant immunoblot assay (RIBA3.0; Ortho Diagnostic Systems) patterns were arbitrarily defined by the manufacturer as the detection of only one antibody out of the four that were sought, namely, c100 (NS4 encoded), c22 (core encoded), c33c (NS3 encoded), and NS5 (NS5 encoded). The aims of the present study were (i) to determine the prevalence of indeterminate RIBA3.0 patterns in patients consecutively tested for anti-HCV antibodies in a university hospital; (ii) to evaluate the significance of these patterns in terms of viral replication, liver disease, and risk factors for HCV; and (iii) to get an insight into the mechanism underlying this peculiar immune response. Among 3,074 serum samples consecutively tested for anti-HCV antibodies, 588 were found to be positive by screening assays. Fifty-nine of them (10%) were RIBA3.0 indeterminate and were compared with 59 RIBA3.0-positive ones. Thirty-one RIBA3.0-indeterminate and 53 RIBA3.0-positive serum samples were HCV RNA positive by PCR (53 versus 90%; P < 10 super(-6)). RIBA3.0-indeterminate and RIBA3.0-positive patients with positive PCR results were not significantly different for the prevalence of risk factors for HCV infection and elevated serum alanine aminotransferase activities. Immunosuppression, attributable to coexisting human immunodeficiency virus infection, organ transplantation, or the administration of immunosuppressive drugs, was significantly more frequent in PCR-positive, RIBA3.0-indeterminate patients than in PCR-negative, RIBA3.0-indeterminate patients (P < 0.001) and PCR-positive patients with a positive RIBA3.0 result (P < 0.01). The distribution of HCV genotypes did not differ significantly between HCV RNA-positive patients with indeterminate or positive RIBA3.0 results. In conclusion, the prevalence of indeterminate RIBA3.0 patterns in virology laboratories is about 10%; in about half of these patients HCV replication is detected by PCR; the main factor responsible for indeterminate RIBA3.0 patterns could be immunosuppression, whereas HCV genotypes do not seem to play a major role.
Indeterminate hepatitis C virus (HCV) third-generation recombinant immunoblot assay (RIBA3.0; Ortho Diagnostic Systems) patterns were arbitrarily defined by the manufacturer as the detection of only one antibody out of the four that were sought, namely, c100 (NS4 encoded), c22 (core encoded), c33c (NS3 encoded), and NS5 (NS5 encoded). The aims of the present study were (i) to determine the prevalence of indeterminate RIBA3.0 patterns in patients consecutively tested for anti-HCV antibodies in a university hospital; (ii) to evaluate the significance of these patterns in terms of viral replication, liver disease, and risk factors for HCV; and (iii) to get an insight into the mechanism underlying this peculiar immune response. Among 3,074 serum samples consecutively tested for anti-HCV antibodies, 588 were found to be positive by screening assays. Fifty-nine of them (10%) were RIBA3.0 indeterminate and were compared with 59 RIBA3.0-positive ones. Thirty-one RIBA3.0-indeterminate and 53 RIBA3.0-positive serum samples were HCV RNA positive by PCR (53 versus 90%; P < 10(-6). RIBA3.0-indeterminate and RIBA-3.0-positive patients with positive PCR results were not significantly different for the prevalence of risk factors for HCV infection and elevated serum alanine aminotransferase activities. Immunosuppression, attributable to coexisting human immunodeficiency virus infection, organ transplantation, or the administration of immunosuppressive drugs, was significantly more frequent in PCR-positive, RIBA3.0-indeterminate patients than in PCR-negative, RIBA3.0 indeterminate patients (P < 0.001) and PCR-positive patients with a positive RIBA3.0 result (P < 0.01). The distribution of HCV genotypes did not differ significantly between HCV RNA-positive patients with indeterminate or positive RIBA3.0 results. In conclusion, the prevalence of indeterminate RIBA3.0 patterns in virology laboratories is about 10%; in about half of these patients HCV replication is detected by PCR; the main factor responsible for indeterminate RIBA3.0 patterns could be immunosuppression, whereas HCV genotypes do not seem to play major role.
Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
Author F Darthuy
J Remire
J Duval
C Sayada
D Dhumeaux
J M Pawlotsky
C Pellet
A Bastie
L Wolfe
AuthorAffiliation Department of Bacteriology and Virology, Hôpital Henri Mondor, Université Paris XII, Créteil, France
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  surname: SAYADA
  fullname: SAYADA, C
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Keywords Human
Antibody
Hepatic disease
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Infection
Virus
Viral disease
Serological method
Digestive diseases
Diagnosis
Flaviviridae
Detection
Hepatitis C virus
Test interpretation
Viral hepatitis C
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Snippet Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
Indeterminate hepatitis C virus (HCV) third-generation recombinant immunoblot assay (RIBA3.0; Ortho Diagnostic Systems) patterns were arbitrarily defined by...
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StartPage 80
SubjectTerms AIDS/HIV
Antigens, Viral - genetics
Biological and medical sciences
Diagnostic Errors
Evaluation Studies as Topic
Genotype
Hepacivirus - genetics
Hepacivirus - immunology
Hepatitis C - diagnosis
Hepatitis C - immunology
Hepatitis C - virology
Hepatitis C Antibodies - blood
hepatitis C virus
Human viral diseases
Humans
Immune Tolerance
Immunoblotting - methods
Immunocompetence
Infectious diseases
Medical sciences
Polymerase Chain Reaction
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Risk Factors
RNA, Viral - genetics
RNA, Viral - isolation & purification
Viral diseases
Viral hepatitis
Virus Replication
Title Significance of indeterminate third-generation hepatitis C virus recombinant immunoblot assay
URI http://jcm.asm.org/content/34/1/80.abstract
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