Increased homologous integration frequency in Yarrowia lipolytica strains defective in non-homologous end-joining

• Published in: Current genetics Vol. 59; no. 1-2; pp. 63 - 72
• Main Authors: Kretzschmar, Anne, Otto, Christina, Holz, Martina, Werner, Severine, Hübner, Linda, Barth, Gerold
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.05.2013
• Subjects: Anti-Bacterial Agents; Anti-Bacterial Agents - pharmacology; antibiotics; Biochemistry; Biomedical and Life Sciences; Cell Biology; classification; DNA; DNA End-Joining Repair; DNA End-Joining Repair - genetics; drug effects; Gene Deletion; genes; genetics; homologous recombination; Homologous Recombination - genetics; Life Sciences; Microbial Genetics and Genomics; Microbial Sensitivity Tests; Microbiology; mutagenicity; Mutagens; Mutagens - pharmacology; Mutation; pharmacology; Plant Sciences; Proteomics; Research Article; ultraviolet radiation; Yarrowia; Yarrowia - classification; Yarrowia - drug effects; Yarrowia - genetics; Yarrowia lipolytica; yeasts; Homologous recombination (HR); Non-homologous end-joining (NHEJ)

The ascomycetous yeast Yarrowia lipolytica has been established as model system for studies of several research topics as well as for biotechnological processes in the last two decades. However, frequency of heterologous recombination is high in this yeast species, and so knockouts of genes are labo...