Lipoxygenase‐mediated generation of lipid peroxides enhances ferroptosis induced by erastin and RSL3

• Published in: Cancer science Vol. 108; no. 11; pp. 2187 - 2194
• Main Authors: Shintoku, Ryosuke, Takigawa, Yuta, Yamada, Keiichi, Kubota, Chisato, Yoshimoto, Yuhei, Takeuchi, Toshiyuki, Koshiishi, Ichiro, Torii, Seiji
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2017; John Wiley and Sons Inc
• Subjects: Apoptosis; Arachidonate 15-Lipoxygenase - genetics; Cancer; Carbolines - administration & dosage; Cell death; Cell Death - drug effects; Cell Line, Tumor; Cell membranes; Fatty acids; Ferroptosis; Fibrosarcoma - drug therapy; Fibrosarcoma - genetics; Fibrosarcoma - pathology; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; glutathione peroxidase; Humans; Immunofluorescence; Immunoglobulins; iron; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Lipoxygenase; Membranes; Original; Oxidation; Pancreatic Neoplasms; Pancreatic Neoplasms - drug therapy; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - pathology; Piperazines - administration & dosage; Ras; Reactive oxygen species; RNA, Small Interfering; siRNA; United States > US; Japan; iron; Cell death; Ras; reactive oxygen species; glutathione peroxidase

In cancer cells the small compounds erastin and RSL3 promote a novel type of cell death called ferroptosis, which requires iron‐dependent accumulation of lipid reactive oxygen species. Here we assessed the contribution of lipid peroxidation activity of lipoxygenases (LOX) to ferroptosis in oncogenic...