Identification of a small-molecule inhibitor of dengue virus using a replicon system

• Published in: Archives of virology Vol. 157; no. 4; pp. 681 - 688
• Main Authors: Hsu, Yu-Chen, Chen, Nai-Chi, Chen, Po-Chiang, Wang, Chun-Chung, Cheng, Wei-Chieh, Wu, Huey-Nan
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.04.2012; Springer; Springer Nature B.V
• Subjects: Animals; Antiviral Agents; Antiviral Agents - chemistry; Antiviral Agents - isolation & purification; Antiviral Agents - pharmacology; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cell Line; chemistry; Dengue fever; Dengue virus; Dengue Virus - drug effects; drug effects; Drug Evaluation, Preclinical; Drug Evaluation, Preclinical - methods; Fundamental and applied biological sciences. Psychology; Genes, Reporter; genetics; Humans; Infectious Diseases; isolation & purification; luciferase; Luciferases; Luciferases - genetics; Luciferases - metabolism; Medical Microbiology; metabolism; methods; Microbiology; Miscellaneous; Molecular biology; Molecular Structure; Original Article; Pathogens; pharmacology; Plasmids; Proteins; public health; replicon; Replicon - drug effects; RNA; screening; Staining and Labeling; subtropics; translation (genetics); Vaccines; Virology; Virus Internalization; Virus Internalization - drug effects; Virus Replication; Virus Replication - drug effects; Viruses; Taiwan; Japanese Encephalitis Virus; Dengue Hemorrhagic Fever; DENV Infection; Dengue Shock Syndrome; Dengue Virus; Virus; Identification; Flaviviridae; Dengue virus; Flavivirus; Dengue group virus

Dengue virus (DENV) is a mosquito-borne human pathogen that causes a serious public-health threat in tropical and subtropical regions of the world. Neither a vaccine to prevent nor an effective therapeutic agent to treat DENV infection is currently available. We established a stable cell line harbor...