Affordable in-house antiretroviral drug resistance assay with good performance in non-subtype B HIV-1

• Published in: Journal of virological methods Vol. 163; no. 2; pp. 505 - 508
• Main Authors: Wallis, Carole L., Papathanasopoulos, Maria A., Lakhi, Shabir, Karita, Etienne, Kamali, Anatoli, Kaleebu, Pontiano, Sanders, Eduard, Anzala, Omu, Bekker, Linda-Gail, Stevens, Gwynn, Wit, Tobias F. Rinke de, Stevens, Wendy
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.02.2010; Elsevier
• Subjects: Affordable; Anti-Retroviral Agents - pharmacology; Antiretroviral drug resistance; Antiviral agents; Automation; Biological and medical sciences; DNA Primers - genetics; Drug Resistance, Viral; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genotype; HIV-1 - drug effects; HIV-1 - genetics; HIV-1 - isolation & purification; HIV-1 subtype C; Human immunodeficiency virus 1; Humans; Microbial Sensitivity Tests - economics; Microbial Sensitivity Tests - methods; Microbiology; Mutation profile; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Viral - genetics; RNA, Viral - isolation & purification; Sensitivity and Specificity; South Africa; Techniques used in virology; Virology; South Africa; Antiretroviral drug resistance; HIV-1 subtype C; Affordable; Mutation profile; Antiretroviral agent; HIV-1 virus; Retroviridae; Method; Lentivirus; Virus; Resistance; Antiviral; Human immunodeficiency virus; Mutation; Subtype

The introduction of antiretroviral (ARV) therapy in resource-poor settings is effective in suppressing HIV-1 replication and prolonging life of infected individuals. This has led to a demand for affordable HIV-1 drug resistance assays, since treatment failure due to development of drug resistance is...