Multiplicity and molecular epidemiology of Plasmodium vivax and Plasmodium falciparum infections in East Africa

• Published in: Malaria journal Vol. 17; no. 1; pp. 185 - 14
• Main Authors: Zhong, Daibin, Lo, Eugenia, Wang, Xiaoming, Yewhalaw, Delenasaw, Zhou, Guofa, Atieli, Harrysone E., Githeko, Andrew, Hemming-Schroeder, Elizabeth, Lee, Ming-Chieh, Afrane, Yaw, Yan, Guiyun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 02.05.2018; BioMed Central; BMC
• Subjects: Adult; Age Factors; Amplicon deep sequencing; Control; Diagnosis; Disease transmission; Epidemiology; Ethiopia - epidemiology; Genetic Variation; Geography; High-Throughput Nucleotide Sequencing; Humans; Kenya - epidemiology; Malaria; Malaria, Falciparum - epidemiology; Malaria, Falciparum - parasitology; Malaria, Vivax - epidemiology; Malaria, Vivax - parasitology; Male; Merozoite surface protein 1; Merozoite Surface Protein 1 - analysis; Molecular Epidemiology; Multiplicity of infection; Patient outcomes; Plasmodium; Plasmodium falciparum - genetics; Plasmodium falciparum - physiology; Plasmodium vivax - genetics; Plasmodium vivax - physiology; Prevalence; Recurrence; Risk factors; Sex Factors; Time Factors; Within-host diversity; Kenya; Ethiopia; Africa; Amplicon deep sequencing; Molecular epidemiology; Plasmodium; Within-host diversity; Multiplicity of infection; Merozoite surface protein 1

Parasite genetic diversity and multiplicity of infection (MOI) affect clinical outcomes, response to drug treatment and naturally-acquired or vaccine-induced immunity. Traditional methods often underestimate the frequency and diversity of multiclonal infections due to technical sensitivity and speci...