Localization of the Tight Junction Protein, ZO-1, Is Modulated by Extracellular Calcium and Cell-Cell Contact in Madin-Darby Canine Kidney Epithelial Cells

• Published in: The Journal of cell biology Vol. 107; no. 6; pp. 2389 - 2399
• Main Authors: Janet D'Angelo Siliciano, Goodenough, Daniel A.
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.12.1988; The Rockefeller University Press
• Subjects: Acinar cells; Antibodies, Monoclonal - immunology; Biological and medical sciences; Calcium; Calcium - pharmacology; Cell Adhesion; Cell culture techniques; Cell Line; Cell lines; Cell membranes; Cell membranes. Ionic channels. Membrane pores; Cell structures and functions; Cells; Chemical suspensions; Cultured cells; Epithelial cells; Epithelium - ultrastructure; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Intercellular Junctions - physiology; Intercellular Junctions - ultrastructure; kidney; Membrane Proteins - immunology; Membrane Proteins - physiology; Molecular and cellular biology; Phosphoproteins - immunology; Phosphoproteins - physiology; Solubility; Tight junctions; Time Factors; Zonula Occludens-1 Protein; Fissipedia; Cell culture; Carnivora; Calcium; Solubility; Environmental factor; Cell cell interaction; Monoclonal antibody; Indirect immunofluorescence; Kidney; Immunological method; Proteins; Vertebrata; Suspension culture; Mammalia; Monolayer culture; Plasma membrane; Immunoblotting assay; Epithelial cell; Tight junction; Localization; Dog

Using the monoclonal antibody R26.4, we have previously identified a ∼225-kD peripheral membrane protein, named ZO-1, that is uniquely associated with the tight junction (zonula occludens) in a variety of epithelia including the Madin-Darby canine kidney (MDCK) epithelial cell line. In this study we...