Terminal RFLP analysis to determine the oral microbiota with hyposalivation

• Published in: Archives of microbiology Vol. 196; no. 7; pp. 489 - 496
• Main Authors: Hayashi, Y, Saito, T, Ohshima, T, Nakagawa, Y, Arita, T, Yashima, A, Makino, T, Konnai, R, Gomi, K, Arai, T, Maeda, N
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.07.2014; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Adult; Aged; Aged, 80 and over; Bacteria; Bacteria - genetics; Bacterial Physiological Phenomena; Biochemistry; Biodiversity; Biomedical and Life Sciences; Biotechnology; Candida albicans; Candida albicans - physiology; Cell Biology; coatings; Dental caries; Dentistry; Dialister; DNA, Ribosomal - genetics; Ecology; Female; Fusobacterium; Gum disease; hemorrhage; Humans; Life Sciences; Male; microbial communities; Microbial Ecology; Microbiology; Microbiota; Microbiota - genetics; Middle Aged; Original Paper; pathogens; Polymorphism; Polymorphism, Restriction Fragment Length; Prevotella; Principal Component Analysis; restriction fragment length polymorphism; ribosomal DNA; saliva; Saliva - chemistry; Saliva - microbiology; Stomatognathic Diseases - microbiology; Streptococcus; Teeth; Tongue; Tongue - microbiology; Veillonella; Xerostomia - microbiology; Japan; Hyposalivation; 16S rDNA; Oral microbiota; T-RFLP

Previous studies of oral microbiota by culture-dependent or targeted DNA approaches demonstrated that hyposalivation, a reduction in salivary secretions, might increase the amount of certain oral pathogens. However, the relationship between hyposalivation and the balance of oral microbiota, especial...