Calcineurin-mediated Dephosphorylation of Synaptotagmin VI Is Necessary for Acrosomal Exocytosis

• Published in: The Journal of biological chemistry Vol. 285; no. 34; pp. 26269 - 26278
• Main Authors: Castillo Bennett, Jimena, Roggero, Carlos M., Mancifesta, Franco E., Mayorga, Luis S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.08.2010; American Society for Biochemistry and Molecular Biology
• Subjects: Acrosome - physiology; Calcineurin; Calcineurin - metabolism; Calcium; Cell Biology; Developmental Biology; Exocytosis; Humans; Male; Membrane Fusion; Membrane Trafficking; Mutation; Phosphorylation - physiology; Protein Phosphatase; Protein Structure, Tertiary; Secretion; Spermatozoa; Spermatozoa - metabolism; Synaptotagmins - genetics; Synaptotagmins - metabolism; Time Factors; Membrane Trafficking; Protein Phosphatase; Spermatozoa; Calcium; Membrane Fusion; Secretion; Calcineurin

Regulated secretion is a fundamental process underlying the function of many cell types. In particular, acrosomal exocytosis in mammalian sperm is essential for egg fertilization. In general, exocytosis is initiated by a cytosolic calcium increase. In this report we show that calcium affects several...