RecO-mediated DNA homology search and annealing is facilitated by SsbA

• Published in: Nucleic acids research Vol. 38; no. 20; pp. 6920 - 6929
• Main Authors: Manfredi, Candela, Suzuki, Yuki, Yadav, Tribhuwan, Takeyasu, Kunio, Alonso, Juan C
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.11.2010
• Subjects: annealing; Bacillus subtilis; Bacillus subtilis - genetics; Bacterial Proteins - metabolism; chemical elements; complementary DNA; DNA - chemistry; DNA - metabolism; DNA, Single-Stranded - chemistry; DNA, Single-Stranded - metabolism; DNA, Single-Stranded - ultrastructure; DNA-Binding Proteins - metabolism; Filamentation; genetic recombination; Genome Integrity, Repair and; Homology; Microscopy, Atomic Force; Nucleoproteins; Plasmids; Protein structure; RecA protein; Recombination; Recombination, Genetic; Secondary structure; Sequence Homology, Nucleic Acid; single-stranded DNA; Transformation

Bacillus subtilis RecO plays a central role in recombinational repair and genetic recombination by (i) stimulating RecA filamentation onto SsbA-coated single-stranded (ss) DNA, (ii) modulating the extent of RecA-mediated DNA strand exchange and (iii) promoting annealing of complementary DNA strands....