Isolation of circulating tumor cells using a microvortex-generating herringbone-chip

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 107; no. 43; pp. 18392 - 18397
• Main Authors: Stott, Shannon L., Hsu, Chia-Hsien, Tsukrov, Dina I., Yu, Min, Miyamoto, David T., Waltman, Belinda A., Rothenberg, S. Michael, Shah, Ajay M., Smas, Malgorzata E., Korir, George K., Floyd, Frederick P., Gilman, Anna J., Lord, Jenna B., Winokur, Daniel, Springer, Simeon, Irimia, Daniel, Nagrath, Sunitha, Sequist, Lecia V., Lee, Richard J., Isselbacher, Kurt J., Maheswaran, Shyamala, Haber, Daniel A., Toner, Mehmet
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 26.10.2010; National Acad Sciences
• Subjects: Base Sequence; Biological Sciences; Biomedical Engineering; Blood; Blood cells; Cancer; Cell Aggregation; Cell Line, Tumor; Cell lines; Cell Separation - instrumentation; Cells; DNA, Neoplasm - genetics; DNA, Neoplasm - isolation & purification; Epithelial cells; Flow velocity; Herringbone; Humans; Imaging; Lung Neoplasms - blood; Male; Metastasis; Microfluidic Analytical Techniques - instrumentation; Neoplastic Cells, Circulating - pathology; Oncogene Proteins, Fusion - genetics; Physical Sciences; Polymerase chain reaction; Prostate cancer; Prostatic Neoplasms - blood; Prostatic Neoplasms - genetics; Prostatic Neoplasms - secondary; Ribonucleic acid; RNA; Tumors

Rare circulating tumor cells (CTCs) present in the bloodstream of patients with cancer provide a potentially accessible source for detection, characterization, and monitoring of nonhematological cancers. We previously demonstrated the effectiveness of a microfluidic device, the CTC-Chip, in capturin...