The Import Competence of a Peroxisomal Membrane Protein Is Determined by Pex19p before the Docking Step

• Published in: The Journal of biological chemistry Vol. 281; no. 45; pp. 34492 - 34502
• Main Authors: Pinto, Manuel P., Grou, Cláudia P., Alencastre, Inês S., Oliveira, Márcia E., Sá-Miranda, Clara, Fransen, Marc, Azevedo, Jorge E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.11.2006; American Society for Biochemistry and Molecular Biology
• Subjects: Adenosine Triphosphate - metabolism; Animals; Cell Membrane - metabolism; CHO Cells; Cricetinae; Green Fluorescent Proteins - genetics; Guanosine Triphosphate - metabolism; Humans; Hydrolysis; Immunoprecipitation; Intracellular Membranes - metabolism; Lipoproteins - immunology; Lipoproteins - metabolism; Liver - cytology; Liver - metabolism; Membrane Proteins - genetics; Membrane Proteins - immunology; Membrane Proteins - metabolism; Molecular Chaperones; Peroxins; Peroxisomes - metabolism; Protein Binding; Protein Transport; Rabbits; Rats; Recombinant Proteins; Repressor Proteins - metabolism; Subcellular Fractions

Biogenesis of the mammalian peroxisomal membrane requires the action of Pex3p and Pex16p, two proteins present in the organelle membrane, and Pex19p, a protein that displays a dual subcellular distribution (peroxisomal and cytosolic). Pex19p interacts with most peroxisomal intrinsic membrane protein...