Chitosan sponges as tissue engineering scaffolds for bone formation

• Published in: Biotechnology letters Vol. 26; no. 13; pp. 1037 - 1041
• Main Authors: Seol, Y.J, Lee, J.Y, Park, Y.J, Lee, Y.M, Young-Ku, Rhyu, I.C, Lee, S.J, Han, S.B, Chung, C.P
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.07.2004; Springer Nature B.V
• Subjects: Animals; Biological and medical sciences; Biological Sciences; Biotechnology; Bone Remodeling; Bones; Calcium; Cells, Cultured; Chitosan; Freeze drying; Fundamental and applied biological sciences. Psychology; Microscopy, Electron, Scanning; Osteoblasts - cytology; Osteoblasts - ultrastructure; Rats; Skull - cytology; Tissue Engineering; Tissue engineering; Porifera; bone formation; Chitosan; Bone; chitosan sponges; osteoblast; Invertebrata

Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 micrometer pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitor...