Chitosan sponges as tissue engineering scaffolds for bone formation
Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 micrometer pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitor...
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| Published in | Biotechnology letters Vol. 26; no. 13; pp. 1037 - 1041 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Dordrecht
Springer
01.07.2004
Springer Nature B.V |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 micrometer pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microgram per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro. |
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| AbstractList | Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 microm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro. Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 micrometer pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microgram per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro. Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 mu m pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10 super(6) (day 1), 11 x 10 super(6) (day 28) and 12 x 10 super(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) mu g per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro. Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 μm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 × 10^sup 6^ (day 1), 11 × 10^sup 6^ (day 28) and 12 × 10^sup 6^ (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) μg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro. [PUBLICATION ABSTRACT] |
| Author | Rhyu, I.C Seol, Y.J Chung, C.P Lee, J.Y Park, Y.J Lee, Y.M Lee, S.J Young-Ku Han, S.B |
| Author_xml | – sequence: 1 fullname: Seol, Y.J – sequence: 2 fullname: Lee, J.Y – sequence: 3 fullname: Park, Y.J – sequence: 4 fullname: Lee, Y.M – sequence: 5 fullname: Young-Ku – sequence: 6 fullname: Rhyu, I.C – sequence: 7 fullname: Lee, S.J – sequence: 8 fullname: Han, S.B – sequence: 9 fullname: Chung, C.P |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15949711$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/15218375$$D View this record in MEDLINE/PubMed |
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| Keywords | Tissue engineering Porifera bone formation Chitosan Bone chitosan sponges osteoblast Invertebrata |
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| PublicationTitle | Biotechnology letters |
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| Snippet | Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a... |
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| SubjectTerms | Animals Biological and medical sciences Biological Sciences Biotechnology Bone Remodeling Bones Calcium Cells, Cultured Chitosan Freeze drying Fundamental and applied biological sciences. Psychology Microscopy, Electron, Scanning Osteoblasts - cytology Osteoblasts - ultrastructure Rats Skull - cytology Tissue Engineering |
| Title | Chitosan sponges as tissue engineering scaffolds for bone formation |
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