Role of Single-stranded DNA in Targeting REV1 to Primer Termini

• Published in: The Journal of biological chemistry Vol. 281; no. 34; pp. 24314 - 24321
• Main Authors: Masuda, Yuji, Kamiya, Kenji
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.08.2006; American Society for Biochemistry and Molecular Biology
• Subjects: Binding Sites; Catalysis; DNA Replication; DNA, Single-Stranded - genetics; DNA, Single-Stranded - metabolism; Escherichia coli; Humans; Mutation; Nuclear Proteins; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; Protein Binding; Protein Structure, Tertiary; Substrate Specificity

Cellular functions of the REV1 gene have been conserved in evolution and appear important for maintaining genetic integrity through translesion DNA synthesis. This study documents a novel biochemical activity of human REV1 protein, due to higher affinity for single-stranded DNA (ssDNA) than the prim...