Deficiency of D‐alanyl‐D‐alanine ligase A attenuated cell division and greatly altered the proteome of Mycobacterium smegmatis

• Published in: MicrobiologyOpen (Weinheim) Vol. 8; no. 9; pp. e00819 - n/a
• Main Authors: Chen, Yingfei, Xu, Yuefei, Yang, Shufeng, Li, Sheng, Ding, Wenyong, Zhang, Wenli
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.09.2019; John Wiley and Sons Inc; Wiley
• Subjects: Alanine; Antibiotics; Antibodies; Antisense RNA; Biodegradation; Biosynthesis; Carbohydrates; Catabolism; Cell division; Cell survival; Cloning; DdlA deficiency; Deoxyribonucleic acid; Dimerization; DNA; D‐alanyl‐D‐alanine ligase A (DdlA); E coli; Electron microscopy; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Fatty acids; Gel electrophoresis; Gene expression; Gene Knockdown Techniques; Mass Spectrometry; Mass spectroscopy; Methanol dehydrogenase; Microscopy, Electrochemical, Scanning; Microscopy, Electron, Transmission; Morphology; Mycobacterium smegmatis; Mycobacterium smegmatis - chemistry; Mycobacterium smegmatis - enzymology; Mycobacterium smegmatis - growth & development; Mycobacterium smegmatis - ultrastructure; N-Nitrosodimethylamine; Original; Peptide Synthases - deficiency; Peptide Synthases - metabolism; Peptidoglycans; Plasmids; Protein biosynthesis; Proteins; proteome; Proteome - analysis; Proteomes; Ribonucleic acid; RNA; Scanning electron microscopy; Studies; Transmission electron microscopy; Tuberculosis; Virulence; China; Mycobacterium smegmatis; cell division; DdlA deficiency; D-alanyl-D-alanine ligase A (DdlA); proteome

D‐Alanyl‐D‐alanine ligase A (DdlA) catalyses the dimerization of two D‐alanines yielding D‐alanyl‐D‐alanine required for mycobacterial peptidoglycan biosynthesis, and is a promising antimycobacterial drug target. To better understand the roles of DdlA in mycobacteria in vivo, we established a cell m...