Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks
Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined neuronal networks possessing a modular architecture on high-d...
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| Published in | Frontiers in neuroscience Vol. 16; p. 943310 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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Frontiers Media S.A
09.01.2023
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| Abstract | Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined neuronal networks possessing a modular architecture on high-density microelectrode arrays (HD-MEAs), a state-of-the-art electrophysiological tool for recording neural activity with high spatial and temporal resolutions. We first established a surface coating protocol using a cell-permissive hydrogel to stably attach a polydimethylsiloxane microfluidic film on the HD-MEA. We then recorded the spontaneous neural activity of the engineered neuronal network, which revealed an important portrait of the engineered neuronal network-modular architecture enhances functional complexity by reducing the excessive neural correlation between spatially segregated modules. The results of this study highlight the impact of HD-MEA recordings combined with cell engineering technologies as a novel tool in neuroscience to constructively assess the structure-function relationships in neuronal networks. |
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| AbstractList | Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined neuronal networks possessing a modular architecture on high-density microelectrode arrays (HD-MEAs), a state-of-the-art electrophysiological tool for recording neural activity with high spatial and temporal resolutions. We first established a surface coating protocol using a cell-permissive hydrogel to stably attach a polydimethylsiloxane microfluidic film on the HD-MEA. We then recorded the spontaneous neural activity of the engineered neuronal network, which revealed an important portrait of the engineered neuronal network–modular architecture enhances functional complexity by reducing the excessive neural correlation between spatially segregated modules. The results of this study highlight the impact of HD-MEA recordings combined with cell engineering technologies as a novel tool in neuroscience to constructively assess the structure-function relationships in neuronal networks. |
| Author | Sato, Shigeo Yamamoto, Hideaki Kato, Hideyuki Tanii, Takashi Sato, Yuya Hirano-Iwata, Ayumi |
| AuthorAffiliation | 1 Research Institute of Electrical Communication, Tohoku University , Sendai , Japan 3 Faculty of Science and Technology, Oita University , Oita , Japan 5 Advanced Institute for Materials Research, Tohoku University , Sendai , Japan 2 Graduate School of Biomedical Engineering, Tohoku University , Sendai , Japan 4 Faculty of Science and Engineering, Waseda University , Tokyo , Japan |
| AuthorAffiliation_xml | – name: 1 Research Institute of Electrical Communication, Tohoku University , Sendai , Japan – name: 4 Faculty of Science and Engineering, Waseda University , Tokyo , Japan – name: 5 Advanced Institute for Materials Research, Tohoku University , Sendai , Japan – name: 3 Faculty of Science and Technology, Oita University , Oita , Japan – name: 2 Graduate School of Biomedical Engineering, Tohoku University , Sendai , Japan |
| Author_xml | – sequence: 1 givenname: Yuya surname: Sato fullname: Sato, Yuya organization: Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan – sequence: 2 givenname: Hideaki surname: Yamamoto fullname: Yamamoto, Hideaki organization: Research Institute of Electrical Communication, Tohoku University, Sendai, Japan – sequence: 3 givenname: Hideyuki surname: Kato fullname: Kato, Hideyuki organization: Faculty of Science and Technology, Oita University, Oita, Japan – sequence: 4 givenname: Takashi surname: Tanii fullname: Tanii, Takashi organization: Faculty of Science and Engineering, Waseda University, Tokyo, Japan – sequence: 5 givenname: Shigeo surname: Sato fullname: Sato, Shigeo organization: Research Institute of Electrical Communication, Tohoku University, Sendai, Japan – sequence: 6 givenname: Ayumi surname: Hirano-Iwata fullname: Hirano-Iwata, Ayumi organization: Advanced Institute for Materials Research, Tohoku University, Sendai, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36699522$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright © 2023 Sato, Yamamoto, Kato, Tanii, Sato and Hirano-Iwata. Copyright © 2023 Sato, Yamamoto, Kato, Tanii, Sato and Hirano-Iwata. 2023 Sato, Yamamoto, Kato, Tanii, Sato and Hirano-Iwata |
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| Keywords | microfluidic devices cell engineering complex networks cultured neuronal network microelectrode array (MEA) |
| Language | English |
| License | Copyright © 2023 Sato, Yamamoto, Kato, Tanii, Sato and Hirano-Iwata. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| SubjectTerms | cell engineering complex networks cultured neuronal network microelectrode array (MEA) microfluidic devices Neuroscience |
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| Title | Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks |
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