Regulation of synthesis of lactosylceramide and long chain bases in normal and familial hypercholesterolemic cultured proximal tubular cells

• Published in: The Journal of biological chemistry Vol. 261; no. 29; pp. 13474 - 13479
• Main Authors: Chatterjee, S, Clarke, K S, Kwiterovich, P O
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 15.10.1986; American Society for Biochemistry and Molecular Biology
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carbon Radioisotopes; Cells, Cultured; Culture Media; Fundamental and applied biological sciences. Psychology; Galactose - metabolism; Glucose - metabolism; Glycosphingolipids - biosynthesis; Humans; Hyperlipoproteinemia Type II - metabolism; Kidney Tubules, Proximal - metabolism; Lactosylceramides - biosynthesis; Lipids; Lipoproteins, HDL - blood; Lipoproteins, LDL - blood; Other biological molecules; Reference Values; Sphingosine; Tritium; Human origin; Cell culture; Long chain; Sphingolipid; Biosynthesis; Normal; Base; Glycolipid; Regulation(control); Renal tubule; Hypercholesterolemia; β-Lipoprotein; Sphingoglycolipid; Biological receptor

We have investigated the effects of lipoproteins on sphingolipid metabolism in proximal renal tubular cells from normal subjects and low density lipoprotein (LDL) receptor-negative homozygous familial hypercholesterolemic subjects employing radioactive precursors, e.g. [3H]serine, [3H]glucose, and [...