Characteristics of Carbapenemase-Producing Klebsiella pneumoniae Isolated in the Intensive Care Unit of the Largest Tertiary Hospital in Bangladesh

• Published in: Frontiers in microbiology Vol. 11; p. 612020
• Main Authors: Okanda, Takashi, Haque, Anwarul, Koshikawa, Takuro, Islam, Amirul, Huda, Qumrul, Takemura, Hiromu, Matsumoto, Tetsuya, Nakamura, Shigeki
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 15.01.2021
• Subjects: Microbiology; NDM-1; NDM-5; OXA-181; OXA-232; PDR; ST147; NDM-1; OXA-181; OXA-232; ST147; NDM-5; PDR
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2020.612020

For addressing the issue of antimicrobial drug resistance in developing countries, it is important to investigate the characteristics of carbapenemase-producing organisms. We aimed to genetically characterize a carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated in the intensive care unit of a tertiary hospital in Bangladesh. The number of CPKP isolates were 43/145 (30%), of which pandrug-resistant (PDR) strains were 14%. These carbapenemases were New Delhi metallo-beta-lactamase (NDM)-1 (53%), NDM-5 (14%), oxacillinase (OXA)-181 (12%), OXA-232 (10%), NDM-5 + OXA-181 (5%), and NDM-5 + OXA-232 (2%). Many CPKP isolates harbored a variety of resistance genes, and the prevalence of 16S rRNA methyltransferase was particularly high (91%). The 43 CPKP isolates were classified into 14 different sequence types (STs), and the common STs were ST34 (26%), ST147 (16%), ST11 (9%), ST14 (9%), ST25 (7%), and ST231 (7%). In this study, PDR strains were of three types, ST147, ST231, and ST14, and their PDR rates were 57, 33, and 25%, respectively. The spread of the antimicrobial drug resistance of CPKP in Bangladesh was identified. In particular, the emergence of PDR is problem, and there may be its spread as a superbug of antimicrobial treatment.