Intracellular transport of endocytosed chylomicron [3H]retinyl ester in rat liver parenchymal cells. Evidence for translocation of a [3H]retinoid from endosomes to endoplasmic reticulum

• Published in: The Journal of biological chemistry Vol. 260; no. 25; pp. 13566 - 13570
• Main Authors: Blomhoff, R, Eskild, W, Kindberg, G M, Prydz, K, Berg, T
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05.11.1985; American Society for Biochemistry and Molecular Biology
• Subjects: alpha-Fetoproteins - metabolism; Analytical, structural and metabolic biochemistry; Animals; Asialoglycoproteins; Biological and medical sciences; Biological Transport; Centrifugation, Density Gradient; Chylomicrons - metabolism; Coenzymes, vitamins; Endocytosis; Endoplasmic Reticulum - metabolism; Fetuins; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Iodine Radioisotopes; Liver - metabolism; Male; Other biological molecules; Rats; Rats, Inbred Strains; Retinoids - metabolism; Tritium; Intracellular transport; Vertebrata; Endocytosis; Mammalia; Chylomicron; Rat; Liver; Rodentia; Vitamin; Parenchyma; Retinoids; Metabolism

The intracellular transport of chylomicron remnants labeled with [3H]retinyl ester was studied in rat liver parenchymal cells by means of subcellular fractionation in Nycodenz and sucrose density gradients. The data presented indicate that endocytosed chylomicron remnant [3H]retinyl ester initially...