In-Depth Molecular Characterization of Neovascular Membranes Suggests a Role for Hyalocyte-to-Myofibroblast Transdifferentiation in Proliferative Diabetic Retinopathy

• Published in: Frontiers in immunology Vol. 12; p. 757607
• Main Authors: Boneva, Stefaniya Konstantinova, Wolf, Julian, Hajdú, Rozina Ida, Prinz, Gabriele, Salié, Henrike, Schlecht, Anja, Killmer, Saskia, Laich, Yannik, Faatz, Henrik, Lommatzsch, Albrecht, Busch, Martin, Bucher, Felicitas, Stahl, Andreas, Böhringer, Daniel, Bengsch, Bertram, Schlunck, Günther, Agostini, Hansjürgen, Lange, Clemens A. K.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 02.11.2021
• Subjects: Adult; Aged; Cell Transdifferentiation; Cells, Cultured; Computational Biology; Diabetic Retinopathy - complications; Diabetic Retinopathy - drug therapy; Diabetic Retinopathy - metabolism; Diabetic Retinopathy - pathology; Drug Repositioning; Endothelial Cells - metabolism; Endothelial Cells - pathology; Epiretinal Membrane - metabolism; Epiretinal Membrane - pathology; Eye Proteins - biosynthesis; Eye Proteins - genetics; Female; Gene Ontology; Humans; hyalocytes; Imatinib Mesylate - therapeutic use; Immunologic Factors - therapeutic use; Immunology; Male; Middle Aged; myofibroblasts; Myofibroblasts - pathology; proliferative diabetic retinopathy (PDR); retinal neovascularization (RNV); Retinal Neovascularization - etiology; Retinal Neovascularization - metabolism; Retinal Neovascularization - pathology; Retinal Perforations - pathology; RNA sequencing; Single-Cell Analysis; Transcriptome; transdifferentiation; Vitreous Body - immunology; Vitreous Body - pathology; Young Adult; RNA sequencing; Imaging Mass Cytometry; proliferative diabetic retinopathy (PDR); transdifferentiation; retinal neovascularization (RNV); myofibroblasts; hyalocytes

Retinal neovascularization (RNV) membranes can lead to a tractional retinal detachment, the primary reason for severe vision loss in end-stage disease proliferative diabetic retinopathy (PDR). The aim of this study was to characterize the molecular, cellular and immunological features of RNV in orde...