Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level

• Published in: Computational and structural biotechnology journal Vol. 19; pp. 4456 - 4471
• Main Authors: Sobhy, Mohamed A., Tehseen, Muhammad, Takahashi, Masateru, Bralić, Amer, De Biasio, Alfredo, Hamdan, Samir M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2021; Research Network of Computational and Structural Biotechnology; Elsevier
• Subjects: active sites; biotechnology; catalytic activity; cryo-electron microscopy; dissociation; DNA Polymerase δ; DNA Replication; enzymatic reactions; Flap endonuclease; fluorescence; Fluorescence enhancement; Fluorescence quenching; FRET; humans; PIFE; Review; Single-molecule fluorescence; DNA Polymerase δ; Single-molecule fluorescence; Flap endonuclease; DNA Replication; FRET; PIFE; Fluorescence enhancement; Fluorescence quenching

[Display omitted] Flap endonuclease 1 (FEN1) is an important component of the intricate molecular machinery for DNA replication and repair. FEN1 is a structure-specific 5′ nuclease that cleaves nascent single-stranded 5′ flaps during the maturation of Okazaki fragments. Here, we review our research...