Cloning and expression of cystatin, a potent cysteine protease inhibitor from the gut of Haemonchus contortus

• Published in: Parasitology Vol. 122; no. 3; pp. 371 - 378
• Main Authors: NEWLANDS, G.F.J., SKUCE, P.J., KNOX, D.P., SMITH, W.D.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.03.2001
• Subjects: Amino Acid Sequence; Animals; Antibodies, Helminth - biosynthesis; Biological and medical sciences; Blotting, Western - veterinary; Cloning; Cloning, Molecular; cystatin; Cystatins - biosynthesis; Cystatins - genetics; cysteine protease inhibitor; Cysteine Proteinase Inhibitors - genetics; Digestive System - chemistry; E coli; Electrophoresis, Polyacrylamide Gel - veterinary; Endopeptidases - chemistry; Endopeptidases - genetics; Endopeptidases - immunology; Feces - parasitology; Fundamental and applied biological sciences. Psychology; gut membrane complex; Haemonchus - chemistry; Haemonchus contortus; Helminth Proteins; Humans; Immune Sera; immunolocalization; Invertebrates; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - genetics; Membrane Glycoproteins - immunology; Molecular Sequence Data; Nemathelminthia. Plathelmintha; nematode parasite; Parasite Egg Count - veterinary; Parasites; Proteinase inhibitors; Recombinant Proteins - immunology; Research article; Sequence Alignment; Sheep; Sheep Diseases - prevention & control; vaccination; Vaccination - veterinary; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Haemonchus contortus; immunolocalization; nematode parasite; gut membrane complex; cystatin; cysteine protease inhibitor; sheep; vaccination; Immunohistochemistry; Host parasite relation; Cystatin; Cysteine endopeptidases; Vaccination; Molecular cloning; Localization; Nematoda; Ungulata; Enzyme; Digestive system; Gut; Enzyme inhibitor; Parasite; Parasitosis; Gene expression; Helminthiasis; Young animal; Infection; Peptidases; Vertebrata; Mammalia; Plasma membrane; Nematode disease; Helmintha; Hydrolases; Sheep; Nemathelminthia; Molecular complex; Artiodactyla; Invertebrata

A cDNA encoding a cysteine protease inhibitor (cystatin) was identified by immunoscreening a Haemonchus contortus cDNA library with antisera from lambs vaccinated with a protective membrane protein complex (H-gal-GP) derived from the gut of the parasite. The cDNA sequence, designated Cys-1, showed s...