High-throughput quantification of antibiotic resistance genes from an urban wastewater treatment plant

• Published in: FEMS microbiology ecology Vol. 92; no. 3; p. fiw014
• Main Authors: Karkman, Antti, Johnson, Timothy A., Lyra, Christina, Stedtfeld, Robert D., Tamminen, Manu, Tiedje, James M., Virta, Marko
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.03.2016
• Subjects: Anti-Bacterial Agents - pharmacology; antibiotic resistance; antibiotic resistance genes; bacteria; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - isolation & purification; Bacterial Proteins - genetics; Drug resistance in microorganisms; Drug Resistance, Bacterial; effluents; Environmental aspects; Gene Transfer, Horizontal; Genetic aspects; Health aspects; horizontal gene transfer; Integrons; oligodeoxyribonucleotides; quantitative analysis; quantitative polymerase chain reaction; sediments; Sewage - microbiology; Sewage disposal plants; sludge; transposases; Waste Water - microbiology; wastewater; wastewater treatment; Water Purification - instrumentation; Finland; qPCR; sewage; sludge

Antibiotic resistance among bacteria is a growing problem worldwide, and wastewater treatment plants have been considered as one of the major contributors to the dissemination of antibiotic resistance to the environment. There is a lack of comprehensive quantitative molecular data on extensive numbe...