Functional analysis of the response regulator DegU in Bacillus megaterium DSM319 and comparative secretome analysis of degSU mutants

• Published in: Applied microbiology and biotechnology Vol. 91; no. 3; pp. 699 - 711
• Main Authors: Borgmeier, Claudia, Voigt, Birgit, Hecker, Michael, Meinhardt, Friedhelm
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.08.2011; Springer; Springer Nature B.V
• Subjects: Amino Acid Sequence; Amylases; Amylases - genetics; Amylases - metabolism; Analysis; Antibiotics; Applied Genetics and Molecular Biotechnology; Bacillus; Bacillus licheniformis; Bacillus megaterium; Bacillus megaterium - genetics; Bacillus megaterium - metabolism; Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacteria; Bacterial Proteins; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Biomedical and Life Sciences; biosynthesis; Biotechnology; Cell culture; cell walls; Chemical synthesis; Cloning; culture media; DNA polymerase; E coli; Enzymes; Extracellular Matrix Proteins; Fundamental and applied biological sciences. Psychology; Gene Knockout Techniques; genetics; Hexosyltransferases; Hexosyltransferases - biosynthesis; Hexosyltransferases - genetics; hypersecretion; levansucrase; Life Sciences; Mass Spectrometry; metabolism; Microbial Genetics and Genomics; Microbiology; Mutants; Mutation; Peptide Hydrolases; Peptide Hydrolases - genetics; Peptide Hydrolases - metabolism; phenotype; Phosphorylation; Plasmids; Polymerase Chain Reaction; Polypeptides; Protein Sorting Signals; Protein Sorting Signals - genetics; proteinases; Proteins; proteolysis; Proteome; Proteomics; Sequence Alignment; Sequence Deletion; Sequence Deletion - genetics; Studies; Two-component system; Extracellular proteome; DegSU; Hypersecretion; Degradative enzymes; Enzyme; Proteome; Functional analysis; Regulator; Bacillaceae; Extracellular; Bacillales; Bacteria; Mutation; Bacillus megaterium; Comparative study

We functionally analysed the two-component regulatory system DegSU (historically SacU) in Bacillus megaterium DSM319 by generating a genetic knock out as well as a sacU32 mutation. The latter—known to cause a hypersecretion phenotype in Bacillus subtilis —had no influence on extracellular protease a...