Comparative Proteomic Analysis Reveals Varying Impact on Immune Responses in Phorbol 12-Myristate-13-Acetate-Mediated THP-1 Monocyte-to-Macrophage Differentiation

• Published in: Frontiers in immunology Vol. 12; p. 679458
• Main Authors: Pinto, Sneha M., Kim, Hera, Subbannayya, Yashwanth, Giambelluca, Miriam S., Bösl, Korbinian, Ryan, Liv, Sharma, Animesh, Kandasamy, Richard K.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 21.06.2021
• Subjects: Biomarkers; Cell Differentiation - immunology; Cell Membrane; Computational Biology - methods; Cytokines - metabolism; functional networks; Gene Expression Profiling; Humans; Immunity; Immunity, Innate; Immunology; Inflammation Mediators - metabolism; innate immune signaling; macrophage; Macrophages - immunology; Macrophages - metabolism; monocyte; Monocytes - immunology; Monocytes - metabolism; pathways; Proteome; Proteomics - methods; Signal Transduction; Tetradecanoylphorbol Acetate - immunology; THP-1 Cells; TLR signaling; Transcriptome; TLR signaling; differentiation; innate immune signaling; macrophage; monocyte; pathways; functional networks

Macrophages are sentinels of the innate immune system, and the human monocytic cell line THP-1 is one of the widely used in vitro models to study inflammatory processes and immune responses. Several monocyte-to-macrophage differentiation protocols exist, with phorbol 12-myristate-13-acetate (PMA) be...