Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles

• Published in: Advanced materials (Weinheim) Vol. 31; no. 33; pp. e1902575 - n/a
• Main Authors: Liu, Ji, Chang, Jin, Jiang, Ying, Meng, Xiandi, Sun, Tianmeng, Mao, Lanqun, Xu, Qiaobing, Wang, Ming
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.08.2019
• Subjects: Animals; Biological Transport; Biomedical materials; Cell Line, Tumor; CRISPR; CRISPR-Associated Protein 9 - genetics; CRISPR/Cas9; Editing; Fluorescence; Gene Editing - methods; Gene Knockdown Techniques - methods; Gene Transfer Techniques; genome editing; Genomes; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; lipid nanoparticles; Lipids; Lipids - chemistry; Materials science; messenger RNA delivery; Mice; Nanoparticles; Nanoparticles - chemistry; Nuclease; Oxidation-Reduction; Proprotein Convertase 9 - genetics; Proprotein Convertase 9 - metabolism; Proteins; RNA, Guide, CRISPR-Cas Systems - administration & dosage; RNA, Guide, CRISPR-Cas Systems - chemistry; RNA, Messenger - administration & dosage; RNA, Messenger - chemistry; genome editing; lipid nanoparticles; messenger RNA delivery; CRISPR/Cas9

A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single‐guide RNA (sgRNA) into the specific cell and organ. An effective and ver...