Genome‐wide patterns of homoeologous gene flow in allotetraploid coffee

• Published in: Applications in plant sciences Vol. 12; no. 4; pp. e11584 - n/a
• Main Authors: Ortiz, Andre J., Sharbrough, Joel
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.07.2024; Wiley
• Subjects: 3-Aminobutyric acid; ABBA‐BABA test; Alleles; Allopolyploidy; allotetraploidy; Cell division; chloroplast; Chloroplasts; Coffea arabica; Coffee; Cytoplasmic inheritance; Enzymes; Extrachromosomal inheritance; Gene flow; Genes; genome; Genomes; homoeologous gene flow; Hybridization; Interspecific hybridization; introgression; Mitochondria; Natural selection; Polyploidy; species; whole‐genome duplication; Coffea arabica; chloroplast; homoeologous gene flow; ABBA‐BABA test; mitochondria; whole‐genome duplication; allopolyploidy
• ISSN: 2168-0450; 2168-0450
• DOI: 10.1002/aps3.11584

Premise Allopolyploidy—a hybridization‐induced whole‐genome duplication event—has been a major driver of plant diversification. The extent to which chromosomes pair with their proper homolog vs. with their homoeolog in allopolyploids varies across taxa, and methods to detect homoeologous gene flow (HGF) are needed to understand how HGF has shaped polyploid lineages. Methods The ABBA‐BABA test represents a classic method for detecting introgression between closely related species, but here we developed a modified use of the ABBA‐BABA test to characterize the extent and direction of HGF in allotetraploid Coffea arabica. Results We found that HGF is abundant in the C. arabica genome, with both subgenomes serving as donors and recipients of variation. We also found that HGF is highly maternally biased in plastid‐targeted—but not mitochondrial‐targeted—genes, as would be expected if plastid–nuclear incompatibilities exist between the two parent species. Discussion Together, our analyses provide a simple framework for detecting HGF and new evidence consistent with selection favoring overwriting of paternally derived alleles by maternally derived alleles to ameliorate plastid–nuclear incompatibilities. Natural selection therefore appears to shape the direction and intensity of HGF in allopolyploid coffee, indicating that cytoplasmic inheritance has long‐term consequences for polyploid lineages.