Simultaneous Quantification of Apolipoprotein A-I and Apolipoprotein B by Liquid-Chromatography–Multiple- Reaction–Monitoring Mass Spectrometry

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 56; no. 12; pp. 1804 - 1813
• Main Authors: Agger, Sean A, Marney, Luke C, Hoofnagle, Andrew N
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for Clinical Chemistry 01.12.2010; Oxford University Press
• Subjects: Analytical, structural and metabolic biochemistry; Apolipoprotein A-I - blood; Apolipoproteins; Apolipoproteins B - blood; Biological and medical sciences; Biomarkers; Biomarkers - blood; Calibration; Chromatography; Chromatography, High Pressure Liquid; Fundamental and applied biological sciences. Psychology; Human subjects; Humans; Immunoassay; Immunoassays; Investigative techniques, diagnostic techniques (general aspects); Ionization; Isotopes; Mass spectrometry; Medical sciences; Molecular biophysics; Nephelometry and Turbidimetry; Peptides; Plasma; Protein Denaturation; Proteins; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Studies; Tandem Mass Spectrometry; Trifluoroethanol; Urea; Apolipoprotein B; Multiple; Surveillance; Clinical biology; Apolipoprotein AI; Quantization; Biochemistry; Liquid chromatography; Molecular biology; Reaction; Mass spectrometry; Quantitative analysis

If liquid-chromatography-multiple-reaction-monitoring mass spectrometry (LC-MRM/MS) could be used in the large-scale preclinical verification of putative biomarkers, it would obviate the need for the development of expensive immunoassays. In addition, the translation of novel biomarkers to clinical...