Analysis of carbohydrate metabolism enzymes and cellular contents of sugars and proteins during spruce somatic embryogenesis suggests a regulatory role of exogenous sucrose in embryo development

• Published in: Journal of experimental botany Vol. 52; no. 365; pp. 2301 - 2311
• Main Authors: Iraqi, Driss, Tremblay, Francine M.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.12.2001; OXFORD UNIVERSITY PRESS
• Subjects: administration & dosage; analysis; beta-Fructofuranosidase; Biological and medical sciences; Biotechnology; Carbohydrate Metabolism; chemical constituents of plants; culture media; embryo (plant); Embryogenesis; Embryos; Enzymes; enzymology; Eukaryotic cell cultures; fructose; Fundamental and applied biological sciences. Psychology; glucose; Glucosyltransferases; Glucosyltransferases - metabolism; Glycoside Hydrolases; Glycoside Hydrolases - metabolism; growth & development; Hexoses; Hexoses - metabolism; In vitro propagation: entire plant regeneration from tissues and cell cultures; invertase; metabolism; Methods. Procedures. Technologies; Picea; Picea - enzymology; Picea - growth & development; Picea - metabolism; Picea glauca; Picea mariana; Plant cells and fungal cells; Plant physiology and development; plant proteins; Plants; Proteins; Proteins - metabolism; Regulation of Growth, Development and Whole Organism Physiology; Seeds; Seeds - enzymology; Seeds - growth & development; Seeds - metabolism; Somatic embryogenesis; Somatic embryos; Starch; Starch - metabolism; Starches; sucrose; Sucrose - administration & dosage; Sucrose - analysis; sucrose phosphate synthase; sucrose synthase; Sugars; tissue culture; Tissue cultures, protoplasts; β-Fructofuranosidase; Glycosyltransferases; Glucose; Sucrose-phosphate synthase; Somatic embryo; Regulation(control); Enzymatic activity; Softwood forest tree; Gymnospermae; Carbohydrate; Chemical concentration; Sucrose synthase; Picea mariana; Embryonic development; Picea glauca; Enzyme; Starch; Transferases; Metabolism; Fructose; Protein; Glycosidases; Hydrolases; Coniferales; Hexosyltransferases; Spermatophyta; O-Glycosidases

Carbohydrate metabolism was investigated during spruce somatic embryogenesis. During the period of maintenance corresponding to the active phase of embryogenic tissue growth, activities of soluble acid invertase and alkaline invertase increased together with cellular glucose and fructose levels. Dur...