Genetic tools for metabolic engineering of Pichia pastoris

• Published in: Engineering Microbiology Vol. 3; no. 4; p. 100094
• Main Authors: Wu, Xiaoyan, Cai, Peng, Yao, Lun, Zhou, Yongjin J
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2023; Elsevier
• Subjects: CRISPR/Cas9; Genetic tools; Metabolic engineering; Pichia pastoris; Review; Metabolic engineering; Pichia pastoris; CRISPR/Cas9; Genetic tools

The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential in producing chemicals from inexpensive feedstocks, which requires efficient genetic engineeri...