Glycoproteomic Probes for Fluorescent Imaging of Fucosylated Glycans in vivo

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 103; no. 33; pp. 12371 - 12376
• Main Authors: Sawa, Masaaki, Hsu, Tsui-Ling, Itoh, Takeshi, Sugiyama, Masakazu, Hanson, Sarah R., Vogt, Peter K., Wong, Chi-Huey
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 15.08.2006; National Acad Sciences
• Subjects: Alkynes; Azides; Biochemistry; Biological Sciences; Cell Line; Cellular biology; Cycloaddition; Fluorescence; Fluorescence microscopy; Fluorescent Dyes - chemistry; Fluorescent Dyes - metabolism; Fucose - chemistry; Fucose - metabolism; Glycoconjugates; Glycoproteins; Glycoproteins - chemistry; Glycoproteins - metabolism; Glycosylation; Humans; Imaging; Labeling; Ligation; Microscopy; Molecular Structure; Physical Sciences; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - metabolism; Proteome - analysis

Glycomics is emerging as a new field for the biology of complex glycoproteins and glycoconjugates. The lack of versatile glycan-labeling methods has presented a major obstacle to visualizing at the cellular level and studying glycoconjugates. To address this issue, we developed a fluorescent labelin...