Purification and primary sequences of the major arginine-containing antifreeze glycopeptides from the fish Eleginus gracilis

• Published in: The Journal of biological chemistry Vol. 261; no. 14; pp. 6384 - 6389
• Main Authors: Burcham, T S, Osuga, D T, Rao, B N, Bush, C A, Feeney, R E
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 15.05.1986; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Aminoacids, peptides. Hormones. Neuropeptides; Analytical, structural and metabolic biochemistry; Animals; Antifreeze Proteins; Arginine - analysis; Biological and medical sciences; Fishes; Fundamental and applied biological sciences. Psychology; Glycoproteins - analysis; Glycoproteins - isolation & purification; Proteins; Vertebrata; Purification; Glycopeptide; Animal; Pisces; Primary structure; Antifreeze protein

An Arg-containing antifreeze glycoprotein from the polar fish Eleginus gracilis was isolated, and the major components were purified to homogeneity. The general protocol for purification was chromatography of serum on DEAE-cellulose, followed by chromatography on a cation exchanger. DEAE-cellulose c...