Actuation of single downstream nodes in growth factor network steers immune cell migration

• Published in: Developmental cell Vol. 58; no. 13; pp. 1170 - 1188.e7
• Main Authors: Pal, Dhiman Sankar, Banerjee, Tatsat, Lin, Yiyan, de Trogoff, Félix, Borleis, Jane, Iglesias, Pablo A., Devreotes, Peter N.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.07.2023
• Subjects: actin cytoskeleton; Animals; biochemical excitability; cancer; Cell Movement - physiology; development; Dictyostelium - metabolism; Humans; immunity; immunotherapy; Intercellular Signaling Peptides and Proteins; leukocytes; Mechanistic Target of Rapamycin Complex 2 - metabolism; metastasis; Mice; mTOR signaling; optogenetics; Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; actin cytoskeleton; development; metastasis; optogenetics; immunotherapy; leukocytes; cancer; immunity; biochemical excitability; mTOR signaling

Ras signaling is typically associated with cell growth, but not direct regulation of motility or polarity. By optogenetically targeting different nodes in the Ras/PI3K/Akt network in differentiated human HL-60 neutrophils, we abruptly altered protrusive activity, bypassing the chemoattractant recept...