Altered sugar donor specificity and catalytic activity of pteridine glycosyltransferases by domain swapping or site-directed mutagenesis

• Published in: BMB reports Vol. 46; no. 1; pp. 37 - 40
• Main Authors: Kim, H.L., Inje University, Kimhae, Republic of korea, Kim, A.H., Inje University, Kimhae, Republic of korea, Park, M.B., Inje University, Kimhae, Republic of korea, Lee, S.W., College of Medicine, Republic of Korea, Park, Y.S., Inje University, Kimhae, Republic of korea
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Biochemistry and Molecular Biology 01.01.2013; 생화학분자생물학회
• Subjects: Amino Acid Sequence; Domain swapping; Domain swapping,Pteridine glycosyltransferase,Substrate specificity,Tetrahydrobiopterin; Glycosyltransferases - chemistry; Glycosyltransferases - genetics; Glycosyltransferases - metabolism; Molecular Sequence Data; MUTACION; Mutagenesis, Site-Directed; MUTATION; Protein Structure, Tertiary; Pteridine glycosyltransferase; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Sequence Homology, Amino Acid; Site-directed mutagenesis; Substrate Specificity; Tetrahydrobiopterin; 화학

CY-007 and CY-049 pteridine glycosyltransferases (PGTs) that differ in sugar donor specificity to catalyze either glucose or xylose transfer to tetrahydrobiopterin were studied here to uncover the structural determinants necessary for the specificity. The importance of the C-terminal domain and its...