Diversity of polyester-degrading bacteria in compost and molecular analysis of a thermoactive esterase from Thermobifida alba AHK119

• Published in: Applied microbiology and biotechnology Vol. 87; no. 2; pp. 771 - 779
• Main Authors: Hu, Xiaoping, Thumarat, Uschara, Zhang, Xian, Tang, Ming, Kawai, Fusako
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.06.2010; Springer; Springer Nature B.V
• Subjects: Actinomycetales; Actinomycetales - enzymology; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - metabolism; Actinomycetes; Amino Acid Sequence; Amino acids; Bacillus; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Bacterial Proteins; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biodegradable materials; Biodegradation; Biodiversity; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Chemical reactions; Chemicals; chemistry; classification; Cloning; Cloning, Molecular; Composting; composts; Conserved sequence; E coli; Environmental Biotechnology; Enzyme Stability; Enzymes; enzymology; Escherichia coli; Esterase; Esterases; Esterases - chemistry; Esterases - genetics; Esterases - metabolism; Esters; Fundamental and applied biological sciences. Psychology; genes; genetics; Hot Temperature; isolation & purification; Life Sciences; Lipase; metabolism; Microbial Genetics and Genomics; Microbiology; Microorganisms; Molecular Sequence Data; Molecular weight; Particle size; Plastics; Polyesters; Polyesters - metabolism; Polyethylene terephthalate; Polymer films; Polymers; Proteins; Sequence Homology, Amino Acid; signal peptide; Soil Microbiology; Studies; Terephthalic acid; Thermobifida alba; triacylglycerol lipase; Aliphatic-aromatic copolyester degraders; Serine hydrolase; strain AHK119; Thermophilic bacteria in compost; Thermoactive esterase; Compost; Enzyme; Aliphatic compound; Thermobifida alba strain AHK119; Serine; Diversity; Ester polymer; Thermophily; Esterases; Degradation; Hydrolases; Bacteria

More than 100 bacterial strains were isolated from composted polyester films and categorized into two groups, Actinomycetes (four genera) and Bacillus (three genera). Of these isolates, Thermobifida alba strain AHK119 (AB298783) was shown to possess the ability to significantly degrade aliphatic-aro...