Infectious Myonecrosis Virus Has a Totivirus-Like, 120-Subunit Capsid, but with Fiber Complexes at the Fivefold Axes

Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image rec...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 105; no. 45; pp. 17526 - 17531
Main Authors Tang, Jinghua, Ochoa, Wendy F., Sinkovits, Robert S., Poulos, Bonnie T., Ghabrial, Said A., Lightner, Donald V., Baker, Timothy S., Nibert, Max L.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 11.11.2008
National Acad Sciences
Subjects
Animals
Aquaculture
Biological Sciences
Capsid
Capsid proteins
Capsid Proteins - genetics
Cryoelectron Microscopy
Dimers
Double stranded RNA
Genome, Viral - genetics
Genomes
Genomics
Marine
Models, Molecular
Penaeidae - virology
Proteins
Ribonucleic acid
RNA
Shellfish
Shrimp
Totiviridae
Totiviridae - genetics
Virion - ultrastructure
Virions
Viruses
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Abstract Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-Å resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 Å in diameter, but with fiber complexes protruding a further 80 Å at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.
AbstractList Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae , it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-Å resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 Å in diameter, but with fiber complexes protruding a further 80 Å at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme. dsRNA virus electron cryomicroscopy nonenveloped virus penaeid shrimp Totiviridae
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-A resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 A in diameter, but with fiber complexes protruding a further 80 A at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme. [PUBLICATION ABSTRACT]
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-A resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 A in diameter, but with fiber complexes protruding a further 80 A at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae , it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-Å resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 Å in diameter, but with fiber complexes protruding a further 80 Å at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-Å resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 Å in diameter, but with fiber complexes protruding a further 80 Å at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-oe... resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 oe... in diameter, but with fiber complexes protruding a further 80 oe... at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.
Author Tang, Jinghua
Lightner, Donald V.
Nibert, Max L.
Sinkovits, Robert S.
Poulos, Bonnie T.
Baker, Timothy S.
Ochoa, Wendy F.
Ghabrial, Said A.
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Author contributions: J.T., W.F.O., R.S.S., B.T.P., D.V.L., T.S.B., and M.L.N. designed research; J.T., W.F.O., R.S.S., B.T.P., D.V.L., T.S.B., and M.L.N. performed research; J.T., R.S.S., B.T.P., D.V.L., and T.S.B. contributed new reagents/analytic tools; J.T., W.F.O., R.S.S., B.T.P., S.A.G., D.V.L., T.S.B., and M.L.N. analyzed data; and J.T., R.S.S., S.A.G., D.V.L., T.S.B., and M.L.N. wrote the paper.
Edited by Reed B. Wickner, National Institutes of Health, Bethesda, MD, and approved September 18, 2008
2Present address: Marine Phage Laboratory, Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ 85721
1Present address: Electron Microscopy Facility, Burnham Institute for Medical Research, La Jolla, CA 92037.
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Snippet Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a...
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae , it has a...
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae , it has a...
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StartPage 17526
SubjectTerms Animals
Aquaculture
Biological Sciences
Capsid
Capsid proteins
Capsid Proteins - genetics
Cryoelectron Microscopy
Dimers
Double stranded RNA
Genome, Viral - genetics
Genomes
Genomics
Marine
Models, Molecular
Penaeidae - virology
Proteins
Ribonucleic acid
RNA
Shellfish
Shrimp
Totiviridae
Totiviridae - genetics
Virion - ultrastructure
Virions
Viruses
Title Infectious Myonecrosis Virus Has a Totivirus-Like, 120-Subunit Capsid, but with Fiber Complexes at the Fivefold Axes
URI https://www.jstor.org/stable/25465312
http://www.pnas.org/content/105/45/17526.abstract
https://www.ncbi.nlm.nih.gov/pubmed/18981418
https://www.proquest.com/docview/201346712/abstract/
https://search.proquest.com/docview/20772353
https://search.proquest.com/docview/69781178
https://pubmed.ncbi.nlm.nih.gov/PMC2582295
Volume 105
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