Optimized simultaneous saccharification and co-fermentation of rice straw for ethanol production by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture using design of experiments

• Published in: Bioresource technology Vol. 142; pp. 171 - 178
• Main Authors: Suriyachai, Nopparat, Weerasaia, Khatiya, Laosiripojana, Navadol, Champreda, Verawat, Unrean, Pornkamol
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.08.2013; Elsevier
• Subjects: agitation; Biological and medical sciences; Biomass; Biotechnology; Co-culture; coculture; Coculture Techniques; Design of experiment; Design of experiments; economic feasibility; Ethanol; Ethanol - metabolism; ethanol production; Ethyl alcohol; experimental design; Fermentation; Food industries; Fundamental and applied biological sciences. Psychology; Mathematical models; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Oryza - metabolism; Rice; rice straw; Saccharification; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Scheffersomyces stipitis; Simultaneous saccharification and co-fermentation (SSCF) optimization; Straw; temperature; Use and upgrading of agricultural and food by-products. Biotechnology; Simultaneous saccharification and co-fermentation (SSCF) optimization; Ethanol; Co-culture; Design of experiment; Ascomycota; Rice straw; Cereal by product; Saccharification; Fermentation; Optimization; Fungi; Experimental design; Saccharomyces cerevisiae
• DOI: 10.1016/j.biortech.2013.05.003
• ISSN: 0960-8524; 1873-2976

•Co-culture SSCF process of rice straw for ethanol production was optimized.•Effect of solid loading on enzyme hydrolysis was examined.•Co-culture was systematically optimized using design of experiment (DoE) approach.•Highly efficient and scalable SSCF by co-culture yielded 99% of theoretical yield.•Maximum ethanol concentration achieved by co-culture SSCF process was 28.6g/L. Herein an ethanol production process from rice straw was optimized. Simultaneous saccharification and co-fermentation (SSCF) using Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture was carried out to enhance ethanol production. The optimal saccharification solid loading was 5%. Key fermentation parameters for co-culture including cell ratio, agitation rate and temperature was rationally optimized using design of experiment (DoE). Optimized co-culture conditions for maximum ethanol production efficiency were at S. cerevisiae:S. stipitis cell ratio of 0.31, agitation rate of 116rpm and temperature of 33.1°C. The optimized SSCF process reached ethanol titer of 15.2g/L and ethanol yield of 99% of theoretical yield, consistent with the DoE model prediction. Moreover, SSCF process under high biomass concentration resulted in high ethanol concentration of 28.6g/L. This work suggests the efficiency and scalability of the developed SSCF process which could provide an important basis for the economic feasibility of ethanol production from lignocelluloses.