Arsenic Directly Binds to and Activates the Yeast AP-1-Like Transcription Factor Yap8

• Published in: Molecular and cellular biology Vol. 36; no. 6; pp. 913 - 922
• Main Authors: Kumar, Nallani Vijay, Yang, Jianbo, Pillai, Jitesh K., Rawat, Swati, Solano, Carlos, Kumar, Abhay, Grøtli, Morten, Stemmler, Timothy L., Rosen, Barry P., Tamás, Markus J.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.03.2016; American Society for Microbiology
• Subjects: Arsenate Reductases - genetics; Arsenic - metabolism; BASIC BIOLOGICAL SCIENCES; Basic-Leucine Zipper Transcription Factors - analysis; Basic-Leucine Zipper Transcription Factors - chemistry; Basic-Leucine Zipper Transcription Factors - metabolism; Biochemistry and Molecular Biology; Biokemi och molekylärbiologi; DNA, Fungal - genetics; DNA, Fungal - metabolism; Gene Expression Regulation, Fungal - drug effects; Membrane Transport Proteins - genetics; Microbiology; Mikrobiologi; Protein Binding; Protein Conformation - drug effects; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - analysis; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Transcription Factor AP-1 - metabolism; Transcriptional Activation - drug effects

The AP-1-like transcription factor Yap8 is critical for arsenic tolerance in the yeast Saccharomyces cerevisiae . However, the mechanism by which Yap8 senses the presence of arsenic and activates transcription of detoxification genes is unknown. Here we demonstrate that Yap8 directly binds to trival...