Docosahexaenoic acid enhances the antioxidant response of human fibroblasts by upregulating γ-glutamyl-cysteinyl ligase and glutathione reductase

• Published in: British journal of nutrition Vol. 95; no. 1; pp. 18 - 26
• Main Authors: Arab, Khelifa, Rossary, Adrien, Flourié, Françoise, Tourneur, Yves, Steghens, Jean-Paul
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.01.2006; Cambridge University Press (CUP)
• Subjects: antioxidant activity; antioxidants; Antioxidants - metabolism; Biological and medical sciences; cell culture; Cells, Cultured; Chemoprevention; Docosahexaenoic acid; Docosahexaenoic Acids - metabolism; Docosahexaenoic Acids - pharmacology; dose response; Dose-Response Relationship, Drug; enzyme activity; Fatty Acids - analysis; Feeding. Feeding behavior; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; free radicals; Fundamental and applied biological sciences. Psychology; Glutamate-Cysteine Ligase - metabolism; Glutathione; glutathione reductase (NADPH); Glutathione Reductase - metabolism; glutathione transferase; Glutathione Transferase - metabolism; Heme Oxygenase (Decyclizing) - metabolism; Humans; Life Sciences; ligases; omega-3 fatty acids; Oxidation-Reduction; Oxidative stress; Oxidative Stress - physiology; Reactive Oxygen Species - analysis; redox reactions; RNA, Messenger - analysis; Sulfhydryl Compounds - metabolism; Thiol-redox status; thiols; Time Factors; Up-Regulation - physiology; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Fibroblasts; Oxidative stress; Docosahexaenoic acid; Chemoprevention; Thiol-redox status; Glutathione; Human; Thiol; Enzyme; Antioxidant; Vertebrata; Mammalia; Ligases; Docosahexaenoic acid: Chemoprevention: Thiol-redox status: Fibroblasts: Glutathione: Oxidative stress; Oxidoreductases; Fibroblast; Reductase

The chemopreventive effects of dietary n-3 PUFA in various pathologies has so far remained controversial, and we were interested in studying their potential influence on cell redox status. DHA (22:6n-3), a typical highly unsaturated n-3 PUFA, was used at 30µmol/l in a model of human fibroblast cell...