Influences of biofluid sample collection and handling procedures on GC–MS based metabolomic studies

• Published in: Journal of bioscience and bioengineering Vol. 110; no. 4; pp. 491 - 499
• Main Authors: Bando, Kiyoko, Kawahara, Rui, Kunimatsu, Takeshi, Sakai, Jun, Kimura, Juki, Funabashi, Hitoshi, Seki, Takaki, Bamba, Takeshi, Fukusaki, Eiichiro
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2010; Osaka, Japan: Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands: Distributed outside Japan by Elsevier Science; Elsevier
• Subjects: Animals; Biological and medical sciences; Biotechnology; Body Fluids; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry - methods; GC–MS; Male; Metabolomics; Plasma; Rats; Rats, Sprague-Dawley; Sample collection; Urine; Urine; Metabolomics; Plasma; Sample collection; GC–MS; GC-MS; Sample

Sample collection procedures of pharmacology and toxicology studies might have a great impact on interpretation of metabolomic study results. Characterization of range variation among sample collection methods is necessary to prevent misinterpretation, as is use of optimal methods in animal experime...