Isolation of plasmid pKM101 in the Stocker laboratory

• Published in: Mutation research Vol. 612; no. 3; pp. 151 - 164
• Main Author: Mortelmans, Kristien
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.06.2006; Amsterdam Elsevier Science; New York, NY
• Subjects: Ames test; Bacterial mutagenesis; Biological and medical sciences; Bruce A.D. Stocker; Carcinogenicity Tests - history; Fundamental and applied biological sciences. Psychology; History, 20th Century; Molecular and cellular biology; Molecular genetics; mucAB; Mutagenesis; Mutagenesis. Repair; Mutagenicity Tests - history; pKM101; Plasmids - genetics; Plasmids - history; Plasmids - isolation & purification; R Factors - genetics; R Factors - history; R Factors - isolation & purification; R plasmids; Salmonella - drug effects; Salmonella - genetics; Salmonella typhimurium; Salmonella/microsome assay; umuDC; Bacterial mutagenesis; R plasmids; pKM101; Ames test; umuDC; Salmonella/microsome assay; mucAB; Bruce A.D. Stocker; Plasmid; Isolation

pKM101 is a mutagenesis-enhancing resistance transfer plasmid (R plasmid) that was introduced into several tester strains used in the Salmonella/microsome mutation assay (Ames test). Plasmid pKM101 has contributed substantially to the effectiveness of the Ames assay, which is used on a world-wide ba...