A size-exclusion-based approach for purifying extracellular vesicles from human plasma

• Published in: Cell reports methods Vol. 1; no. 3; p. 100055
• Main Authors: Vanderboom, Patrick M., Dasari, Surendra, Ruegsegger, Gregory N., Pataky, Mark W., Lucien, Fabrice, Heppelmann, Carrie Jo, Lanza, Ian R., Nair, K. Sreekumaran
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 26.07.2021; Elsevier
• Subjects: bioinformatics; Chromatography, Gel; Extracellular Vesicles - chemistry; high-intensity exercise; Humans; mass spectrometry; Mass Spectrometry - methods; Proteins - analysis; Proteomics - methods; size-exclusion chromatography; ultracentrifugation; mass spectrometry; high-intensity exercise; size-exclusion chromatography; ultracentrifugation; bioinformatics

Extracellular vesicles (EVs) are released into blood from multiple organs and carry molecular cargo that facilitates inter-organ communication and an integrated response to physiological and pathological stimuli. Interrogation of the protein cargo of EVs is currently limited by the absence of optima...