Calcineurin, a type 2B protein phosphatase, modulates the Ca2+-permeable slow vacuolar ion channel of stomatal guard cells

• Published in: The Plant cell Vol. 7; no. 9; pp. 1473 - 1483
• Main Authors: Allen, G.J. (University of York, York, UK.), Sanders, D
• Format: Journal Article
• Language: English
• Published: United States 01.09.1995
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; CALCIO; CALCIUM; CATION; CATIONES; CELLULE; CELULAS; chlorides; CHLORURE; CLORUROS; electrophysiology; enzyme activity; ESTERASAS; ESTERASE; ESTOMA; guard cells; inorganic ions; ION; ion transport; IONES; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; phosphoprotein phosphatase; POTASIO; POTASSIUM; stomata; STOMATE; tonoplast; VACUOLA; VACUOLE; vacuoles; VICIA FABA

The slowly activating vacuolar (SV) channel of plant vacuoles is gated open by cytosolic free Ca2+ and by cytosol-positive potentials. Using vacuoles isolated from broad bean guard cell protoplasts, SV-mediated currents could be measured in the whole-vacuole configuration of a patch clamp as the tim...