Potency Analysis of Mesenchymal Stromal Cells Using a Combinatorial Assay Matrix Approach

• Published in: Cell reports (Cambridge) Vol. 22; no. 9; pp. 2504 - 2517
• Main Authors: Chinnadurai, Raghavan, Rajan, Devi, Qayed, Muna, Arafat, Dalia, Garcia, Marco, Liu, Yifei, Kugathasan, Subra, Anderson, Larry J., Gibson, Greg, Galipeau, Jacques
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 27.02.2018; Elsevier
• Subjects: assay matrix; Cell Communication - drug effects; Cell Proliferation - drug effects; Coculture Techniques; Cytokines - metabolism; Humans; Indoleamine-Pyrrole 2,3,-Dioxygenase - metabolism; Interferon-gamma - pharmacology; interferon-γ; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; mesenchymal stromal cells; PBMCs; secretome; T-Lymphocytes - cytology; T-Lymphocytes - drug effects; transcriptome; interferon-γ; assay matrix; secretome; PBMCs; transcriptome; mesenchymal stromal cells

Assays that can characterize MSC immune potency need to be identified for use in advanced clinical trials. MSCs possess a number of putative regenerative and immunomodulatory properties, and an assay matrix approach may best capture involved effector pathways. We have tested two assay systems to mea...