Methylation status of differentially methylated regions at Igf2/H19 locus in porcine gametes and preimplantation embryos
The aim of this study was to demonstrate how differential methylation imprints are established during porcine preimplantation embryo development. For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences. The methylation marks o...
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| Published in | Genomics (San Diego, Calif.) Vol. 93; no. 2; pp. 179 - 186 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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01.02.2009
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| Abstract | The aim of this study was to demonstrate how differential methylation imprints are established during porcine preimplantation embryo development. For the methylation analysis, the primers for the three
Igf2/H19 DMRs were designed and based upon previously published sequences. The methylation marks of
Igf2/H19 DMRs were analysed in sperm and MII oocytes with our results showing that these regions are fully methylated in sperm but remain unmethylated in MII oocytes. In order to identify the methylation pattern at the pronuclear stage, we indirectly compared the methylation profile of
Igf2/H19 DMR3 in each zygote derived by
in vitro fertilization, parthenogenesis, and androgenesis. Interestingly, this region was found to be differently methylated according to parental origins; DMR3 was hemimethylated in
in vitro fertilized zygotes, fully methylated in parthenogenetic zygotes, and demethylated in androgenetic zygotes. These results indicate that the methylation mark of the paternal allele is erased by active demethylation, and that of the maternal one is
de novo methylated. We further examined the methylation imprints of
Igf2/H19 DMR3 during early embryonic development. The hemimethylated pattern as seen in zygotes fertilized
in vitro was observed up to the 4-cell embryo stage. However, this mark was exclusively demethylated at the 8-cell stage and then restored at the morula stage. These results suggest that methylation imprints are established via dynamic changes during early embryonic development in porcine embryos. |
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| AbstractList | The aim of this study was to demonstrate how differential methylation imprints are established during porcine preimplantation embryo development. For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences. The methylation marks of Igf2/H19 DMRs were analysed in sperm and MII oocytes with our results showing that these regions are fully methylated in sperm but remain unmethylated in MII oocytes. In order to identify the methylation pattern at the pronuclear stage, we indirectly compared the methylation profile of Igf2/H19 DMR3 in each zygote derived by in vitro fertilization, parthenogenesis, and androgenesis. Interestingly, this region was found to be differently methylated according to parental origins; DMR3 was hemimethylated in in vitro fertilized zygotes, fully methylated in parthenogenetic zygotes, and demethylated in androgenetic zygotes. These results indicate that the methylation mark of the paternal allele is erased by active demethylation, and that of the maternal one is de novo methylated. We further examined the methylation imprints of Igf2/H19 DMR3 during early embryonic development. The hemimethylated pattern as seen in zygotes fertilized in vitro was observed up to the 4-cell embryo stage. However, this mark was exclusively demethylated at the 8-cell stage and then restored at the morula stage. These results suggest that methylation imprints are established via dynamic changes during early embryonic development in porcine embryos. The aim of this study was to demonstrate how differential methylation imprints are established during porcine preimplantation embryo development. For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences. The methylation marks of Igf2/H19 DMRs were analysed in sperm and MII oocytes with our results showing that these regions are fully methylated in sperm but remain unmethylated in MII oocytes. In order to identify the methylation pattern at the pronuclear stage, we indirectly compared the methylation profile of Igf2/H19 DMR3 in each zygote derived by in vitro fertilization, parthenogenesis, and androgenesis. Interestingly, this region was found to be differently methylated according to parental origins; DMR3 was hemimethylated in in vitro fertilized zygotes, fully methylated in parthenogenetic zygotes, and demethylated in androgenetic zygotes. These results indicate that the methylation mark of the paternal allele is erased by active demethylation, and that of the maternal one is de novo methylated. We further examined the methylation imprints of Igf2/H19 DMR3 during early embryonic development. The hemimethylated pattern as seen in zygotes fertilized in vitro was observed up to the 4-cell embryo stage. However, this mark was exclusively demethylated at the 8-cell stage and then restored at the morula stage. These results suggest that methylation imprints are established via dynamic changes during early embryonic development in porcine embryos. |
| Author | Park, Chi-Hun Lee, Sang-Goo Lee, Chang-Kyu Kim, Hye-Sun |
| Author_xml | – sequence: 1 givenname: Chi-Hun surname: Park fullname: Park, Chi-Hun – sequence: 2 givenname: Hye-Sun surname: Kim fullname: Kim, Hye-Sun – sequence: 3 givenname: Sang-Goo surname: Lee fullname: Lee, Sang-Goo – sequence: 4 givenname: Chang-Kyu surname: Lee fullname: Lee, Chang-Kyu email: leeck@snu.ac.kr |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21123888$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/18983907$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1074/jbc.M106516200 10.1002/mrd.20728 10.1073/pnas.93.13.6371 10.1016/S0955-0674(03)00031-0 10.1016/j.ygeno.2006.03.011 10.1016/S0167-4781(01)00190-7 10.1126/science.1064020 10.1002/mrd.20620 10.1128/MCB.26.4.1245-1258.2006 10.1093/hmg/ddg315 10.1016/j.ygeno.2007.10.005 10.1093/nar/22.15.2990 10.1093/molehr/gah230 10.1016/j.ygeno.2004.08.012 10.1095/biolreprod62.6.1526 10.1159/000090808 10.1101/gad.14.15.1908 10.1101/gr.161600 10.1128/MCB.17.8.4322 10.1126/science.1063443 10.1093/humupd/7.4.419 10.1016/S1046-2023(02)00060-9 10.1016/S0960-9822(00)00448-6 10.1016/j.ygeno.2005.10.008 10.1002/bdrc.20039 10.1038/nature02402 10.1073/pnas.241522698 10.1002/j.1460-2075.1993.tb06041.x 10.1016/j.anireprosci.2006.10.006 10.1093/nar/29.13.e65 10.1073/pnas.89.5.1827 10.1093/hmg/10.26.2983 10.1095/biolreprod66.3.635 10.1007/s00335-001-3059-x 10.1093/hmg/ddl237 10.1006/geno.1998.5371 10.1007/s00418-006-0153-x 10.1095/biolreprod.105.046284 10.1002/jcb.10684 10.1006/geno.2002.6732 10.1095/biolreprod.103.017293 10.1038/ng1197-275 10.1038/362751a0 10.1002/mrd.1080340107 |
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| Keywords | Parthenogenesis Androgenesis Preimplantation Imprinting DNA methylation Embryonic development Embryo Genomics Blastocyst Insulin like growth factor 2 Genomic imprinting Pig Gamete Vertebrata Mammalia DNA Artiodactyla Locus Methylation Ungulata |
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| References | Fulka, Fulka, Slavik, Okada, Fulka (bib37) 2006; 126 Liu (bib39) 2008; 91 Wylie, Murphy, Orton, Jirtle (bib23) 2000; 10 Tremblay, Duran, Bartolomei (bib8) 1997; 17 Yang (bib7) 2003; 90 Borghol, Lornage, Blachere, Sophie Garret, Lefevre (bib12) 2006; 87 Li, Lees-Murdock, Xu, Walsh (bib21) 2004; 84 Ferguson-Smith, Surani (bib3) 2001; 293 Gebert (bib15) 2006; 88 Amarger (bib22) 2002; 13 Zhu (bib35) 2002; 66 Mann (bib17) 2003; 69 Shemer (bib32) 1996; 93 Frommer (bib24) 1992; 89 Reik, Dean, Walter (bib30) 2001; 293 Geuns, De Rycke, Van Steirteghem, Liebaers (bib14) 2003; 12 Kang (bib19) 2001; 276 Brandeis (bib38) 1993; 12 Thorvaldsen, Fedoriw, Nguyen, Bartolomei (bib9) 2006; 26 Lee (bib43) 2007; 74 Kono (bib2) 2004; 428 Oswald (bib31) 2000; 10 Grunau, Clark, Rosenthal (bib25) 2001; 29 Li (bib18) 2005; 11 Ribas (bib41) 2006; 74 Warnecke (bib26) 2002; 27 Ferguson-Smith, Sasaki, Cattanach, Surani (bib5) 1993; 362 Krisher, Brad, Herrick, Sparman, Swain (bib29) 2007; 98 Hamatani (bib28) 2001; 1518 Kono, Sotomaru, Sato, Nakahara (bib44) 1993; 34 Dean (bib36) 2001; 98 Miyoshi, Barton, Kaneda, Hajkova, Surani (bib4) 2006; 113 Arnaud, Feil (bib16) 2005; 75 Barton (bib33) 2001; 10 Olek, Walter (bib10) 1997; 17 Lucifero, Mertineit, Clarke, Bestor, Trasler (bib13) 2002; 79 Howlett, Reik, Barton, Norris, Surani (bib1) 1989; 6 Kang, Lee, Han (bib20) 2003; 15 Kaffer (bib6) 2000; 14 Engel, Thorvaldsen, Bartolomei (bib27) 2006; 15 Isom, Prather, Rucker (bib34) 2007; 74 Doherty, Mann, Tremblay, Bartolomei, Schultz (bib40) 2000; 62 Warnecke, Mann, Frommer, Clark (bib11) 1998; 51 Clark, Harrison, Paul, Frommer (bib45) 1994; 22 Khosla, Dean, Reik, Feil (bib42) 2001; 7 Borghol (10.1016/j.ygeno.2008.10.002_bib12) 2006; 87 Yang (10.1016/j.ygeno.2008.10.002_bib7) 2003; 90 Grunau (10.1016/j.ygeno.2008.10.002_bib25) 2001; 29 Olek (10.1016/j.ygeno.2008.10.002_bib10) 1997; 17 Krisher (10.1016/j.ygeno.2008.10.002_bib29) 2007; 98 Frommer (10.1016/j.ygeno.2008.10.002_bib24) 1992; 89 Lucifero (10.1016/j.ygeno.2008.10.002_bib13) 2002; 79 Khosla (10.1016/j.ygeno.2008.10.002_bib42) 2001; 7 Warnecke (10.1016/j.ygeno.2008.10.002_bib26) 2002; 27 Zhu (10.1016/j.ygeno.2008.10.002_bib35) 2002; 66 Clark (10.1016/j.ygeno.2008.10.002_bib45) 1994; 22 Mann (10.1016/j.ygeno.2008.10.002_bib17) 2003; 69 Li (10.1016/j.ygeno.2008.10.002_bib21) 2004; 84 Isom (10.1016/j.ygeno.2008.10.002_bib34) 2007; 74 Geuns (10.1016/j.ygeno.2008.10.002_bib14) 2003; 12 Dean (10.1016/j.ygeno.2008.10.002_bib36) 2001; 98 Miyoshi (10.1016/j.ygeno.2008.10.002_bib4) 2006; 113 Wylie (10.1016/j.ygeno.2008.10.002_bib23) 2000; 10 Hamatani (10.1016/j.ygeno.2008.10.002_bib28) 2001; 1518 Lee (10.1016/j.ygeno.2008.10.002_bib43) 2007; 74 Kang (10.1016/j.ygeno.2008.10.002_bib20) 2003; 15 Kono (10.1016/j.ygeno.2008.10.002_bib44) 1993; 34 Amarger (10.1016/j.ygeno.2008.10.002_bib22) 2002; 13 Kaffer (10.1016/j.ygeno.2008.10.002_bib6) 2000; 14 Kang (10.1016/j.ygeno.2008.10.002_bib19) 2001; 276 Li (10.1016/j.ygeno.2008.10.002_bib18) 2005; 11 Tremblay (10.1016/j.ygeno.2008.10.002_bib8) 1997; 17 Oswald (10.1016/j.ygeno.2008.10.002_bib31) 2000; 10 Howlett (10.1016/j.ygeno.2008.10.002_bib1) 1989; 6 Barton (10.1016/j.ygeno.2008.10.002_bib33) 2001; 10 Kono (10.1016/j.ygeno.2008.10.002_bib2) 2004; 428 Shemer (10.1016/j.ygeno.2008.10.002_bib32) 1996; 93 Liu (10.1016/j.ygeno.2008.10.002_bib39) 2008; 91 Engel (10.1016/j.ygeno.2008.10.002_bib27) 2006; 15 Arnaud (10.1016/j.ygeno.2008.10.002_bib16) 2005; 75 Gebert (10.1016/j.ygeno.2008.10.002_bib15) 2006; 88 Warnecke (10.1016/j.ygeno.2008.10.002_bib11) 1998; 51 Brandeis (10.1016/j.ygeno.2008.10.002_bib38) 1993; 12 Fulka (10.1016/j.ygeno.2008.10.002_bib37) 2006; 126 Ribas (10.1016/j.ygeno.2008.10.002_bib41) 2006; 74 Ferguson-Smith (10.1016/j.ygeno.2008.10.002_bib5) 1993; 362 Doherty (10.1016/j.ygeno.2008.10.002_bib40) 2000; 62 Reik (10.1016/j.ygeno.2008.10.002_bib30) 2001; 293 Ferguson-Smith (10.1016/j.ygeno.2008.10.002_bib3) 2001; 293 Thorvaldsen (10.1016/j.ygeno.2008.10.002_bib9) 2006; 26 |
| References_xml | – volume: 293 start-page: 1086 year: 2001 end-page: 1089 ident: bib3 article-title: Imprinting and the epigenetic asymmetry between parental genomes publication-title: Science contributor: fullname: Surani – volume: 10 start-page: 475 year: 2000 end-page: 478 ident: bib31 article-title: Active demethylation of the paternal genome in the mouse zygote publication-title: Curr. Biol. contributor: fullname: Oswald – volume: 10 start-page: 2983 year: 2001 end-page: 2987 ident: bib33 article-title: Genome-wide methylation patterns in normal and uniparental early mouse embryos publication-title: Hum. Mol. Genet. contributor: fullname: Barton – volume: 7 start-page: 419 year: 2001 end-page: 427 ident: bib42 article-title: Culture of preimplantation embryos and its long-term effects on gene expression and phenotype publication-title: Hum. Reprod. Update contributor: fullname: Feil – volume: 51 start-page: 182 year: 1998 end-page: 190 ident: bib11 article-title: Bisulfite sequencing in preimplantation embryos: DNA methylation profile of the upstream region of the mouse imprinted H19 gene publication-title: Genomics contributor: fullname: Clark – volume: 75 start-page: 81 year: 2005 end-page: 97 ident: bib16 article-title: Epigenetic deregulation of genomic imprinting in human disorders and following assisted reproduction publication-title: Birth Defects Res. C Embryo Today contributor: fullname: Feil – volume: 11 start-page: 631 year: 2005 end-page: 640 ident: bib18 article-title: IVF results in de novo DNA methylation and histone methylation at an Igf2-H19 imprinting epigenetic switch publication-title: Mol. Hum. Reprod. contributor: fullname: Li – volume: 362 start-page: 751 year: 1993 end-page: 755 ident: bib5 article-title: Parental-origin-specific epigenetic modification of the mouse H19 gene publication-title: Nature contributor: fullname: Surani – volume: 29 start-page: E65 year: 2001 end-page: 5 ident: bib25 article-title: Bisulfite genomic sequencing: systematic investigation of critical experimental parameters publication-title: Nucleic Acids Res. contributor: fullname: Rosenthal – volume: 98 start-page: 13734 year: 2001 end-page: 13738 ident: bib36 article-title: Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Dean – volume: 90 start-page: 1038 year: 2003 end-page: 1055 ident: bib7 article-title: Epigenetic regulation of Igf2/H19 imprinting at CTCF insulator binding sites publication-title: J. Cell Biochem. contributor: fullname: Yang – volume: 98 start-page: 72 year: 2007 end-page: 96 ident: bib29 article-title: A comparative analysis of metabolism and viability in porcine oocytes during in vitro maturation publication-title: Anim. Reprod. Sci. contributor: fullname: Swain – volume: 293 start-page: 1089 year: 2001 end-page: 1093 ident: bib30 article-title: Epigenetic reprogramming in mammalian development publication-title: Science contributor: fullname: Walter – volume: 12 start-page: 3669 year: 1993 end-page: 3677 ident: bib38 article-title: The ontogeny of allele-specific methylation associated with imprinted genes in the mouse publication-title: EMBO J. contributor: fullname: Brandeis – volume: 113 start-page: 6 year: 2006 end-page: 11 ident: bib4 article-title: The continuing quest to comprehend genomic imprinting publication-title: Cytogenet. Genome Res. contributor: fullname: Surani – volume: 87 start-page: 417 year: 2006 end-page: 426 ident: bib12 article-title: Epigenetic status of the H19 locus in human oocytes following in vitro maturation publication-title: Genomics contributor: fullname: Lefevre – volume: 1518 start-page: 137 year: 2001 end-page: 144 ident: bib28 article-title: Epigenetic mark sequence of the H19 gene in human sperm publication-title: Biochim. Biophys. Acta. contributor: fullname: Hamatani – volume: 34 start-page: 43 year: 1993 end-page: 46 ident: bib44 article-title: Development of androgenetic mouse embryos produced by in vitro fertilization of enucleated oocytes publication-title: Mol. Reprod. Dev. contributor: fullname: Nakahara – volume: 17 start-page: 275 year: 1997 end-page: 276 ident: bib10 article-title: The pre-implantation ontogeny of the H19 methylation imprint publication-title: Nat. Genet. contributor: fullname: Walter – volume: 89 start-page: 1827 year: 1992 end-page: 1831 ident: bib24 article-title: A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Frommer – volume: 17 start-page: 4322 year: 1997 end-page: 4329 ident: bib8 article-title: A 5′ 2-kilobase-pair region of the imprinted mouse H19 gene exhibits exclusive paternal methylation throughout development publication-title: Mol. Cell Biol. contributor: fullname: Bartolomei – volume: 276 start-page: 39980 year: 2001 end-page: 39984 ident: bib19 article-title: Typical demethylation events in cloned pig embryos. Clues on species-specific differences in epigenetic reprogramming of a cloned donor genome publication-title: J. Biol. Chem. contributor: fullname: Kang – volume: 14 start-page: 1908 year: 2000 end-page: 1919 ident: bib6 article-title: A transcriptional insulator at the imprinted H19/Igf2 locus publication-title: Genes Dev. contributor: fullname: Kaffer – volume: 27 start-page: 101 year: 2002 end-page: 107 ident: bib26 article-title: Identification and resolution of artifacts in bisulfite sequencing publication-title: Methods contributor: fullname: Warnecke – volume: 26 start-page: 1245 year: 2006 end-page: 1258 ident: bib9 article-title: Developmental profile of H19 differentially methylated domain (DMD) deletion alleles reveals multiple roles of the DMD in regulating allelic expression and DNA methylation at the imprinted H19/Igf2 locus publication-title: Mol. Cell Biol. contributor: fullname: Bartolomei – volume: 15 start-page: 290 year: 2003 end-page: 295 ident: bib20 article-title: Reprogramming DNA methylation in the preimplantation stage: peeping with Dolly's eyes publication-title: Curr. Opin. Cell Biol. contributor: fullname: Han – volume: 91 start-page: 121 year: 2008 end-page: 128 ident: bib39 article-title: Diploid parthenogenetic embryos adopt a maternal-type methylation pattern on both sets of maternal chromosomes publication-title: Genomics contributor: fullname: Liu – volume: 74 start-page: 307 year: 2006 end-page: 313 ident: bib41 article-title: Effect of zona pellucida removal on DNA methylation in early mouse embryos publication-title: Biol. Reprod. contributor: fullname: Ribas – volume: 12 start-page: 2873 year: 2003 end-page: 2879 ident: bib14 article-title: Methylation imprints of the imprint control region of the SNRPN-gene in human gametes and preimplantation embryos publication-title: Hum. Mol. Genet. contributor: fullname: Liebaers – volume: 79 start-page: 530 year: 2002 end-page: 538 ident: bib13 article-title: Methylation dynamics of imprinted genes in mouse germ cells publication-title: Genomics contributor: fullname: Trasler – volume: 126 start-page: 213 year: 2006 end-page: 217 ident: bib37 article-title: DNA methylation pattern in pig in vivo produced embryos publication-title: Histochem. Cell Biol. contributor: fullname: Fulka – volume: 22 start-page: 2990 year: 1994 end-page: 2997 ident: bib45 article-title: High sensitivity mapping of methylated cytosines publication-title: Nucleic Acids Res. contributor: fullname: Frommer – volume: 74 start-page: 1436 year: 2007 end-page: 1445 ident: bib43 article-title: In vitro development and cell allocation of porcine blastocysts derived by aggregation of in vitro fertilized embryos publication-title: Mol. Reprod. Dev. contributor: fullname: Lee – volume: 10 start-page: 1711 year: 2000 end-page: 1718 ident: bib23 article-title: Novel imprinted DLK1/GTL2 domain on human chromosome 14 contains motifs that mimic those implicated in IGF2/H19 regulation publication-title: Genome Res. contributor: fullname: Jirtle – volume: 88 start-page: 222 year: 2006 end-page: 229 ident: bib15 article-title: The bovine IGF2 gene is differentially methylated in oocyte and sperm DNA publication-title: Genomics contributor: fullname: Gebert – volume: 74 start-page: 574 year: 2007 end-page: 581 ident: bib34 article-title: Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos publication-title: Mol. Reprod. Dev. contributor: fullname: Rucker – volume: 62 start-page: 1526 year: 2000 end-page: 1535 ident: bib40 article-title: Differential effects of culture on imprinted H19 expression in the preimplantation mouse embryo publication-title: Biol. Reprod. contributor: fullname: Schultz – volume: 69 start-page: 902 year: 2003 end-page: 914 ident: bib17 article-title: Disruption of imprinted gene methylation and expression in cloned preimplantation stage mouse embryos publication-title: Biol. Reprod. contributor: fullname: Mann – volume: 13 start-page: 388 year: 2002 end-page: 398 ident: bib22 article-title: Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs publication-title: Mamm. Genome contributor: fullname: Amarger – volume: 428 start-page: 860 year: 2004 end-page: 864 ident: bib2 article-title: Birth of parthenogenetic mice that can develop to adulthood publication-title: Nature contributor: fullname: Kono – volume: 66 start-page: 635 year: 2002 end-page: 641 ident: bib35 article-title: Improvement of an electrical activation protocol for porcine oocytes publication-title: Biol. Reprod. contributor: fullname: Zhu – volume: 93 start-page: 6371 year: 1996 end-page: 6376 ident: bib32 article-title: Dynamic methylation adjustment and counting as part of imprinting mechanisms publication-title: Proc. Natl. Acad. Sci. U. S. A. contributor: fullname: Shemer – volume: 84 start-page: 952 year: 2004 end-page: 960 ident: bib21 article-title: Timing of establishment of paternal methylation imprints in the mouse publication-title: Genomics contributor: fullname: Walsh – volume: 15 start-page: 2945 year: 2006 end-page: 2954 ident: bib27 article-title: CTCF binding sites promote transcription initiation and prevent DNA methylation on the maternal allele at the imprinted H19/Igf2 locus publication-title: Hum. Mol. Genet. contributor: fullname: Bartolomei – volume: 6 start-page: 59 year: 1989 end-page: 77 ident: bib1 article-title: Genomic imprinting in the mouse publication-title: Dev. Biol. contributor: fullname: Surani – volume: 276 start-page: 39980 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib19 article-title: Typical demethylation events in cloned pig embryos. Clues on species-specific differences in epigenetic reprogramming of a cloned donor genome publication-title: J. Biol. Chem. doi: 10.1074/jbc.M106516200 contributor: fullname: Kang – volume: 74 start-page: 1436 year: 2007 ident: 10.1016/j.ygeno.2008.10.002_bib43 article-title: In vitro development and cell allocation of porcine blastocysts derived by aggregation of in vitro fertilized embryos publication-title: Mol. Reprod. Dev. doi: 10.1002/mrd.20728 contributor: fullname: Lee – volume: 93 start-page: 6371 year: 1996 ident: 10.1016/j.ygeno.2008.10.002_bib32 article-title: Dynamic methylation adjustment and counting as part of imprinting mechanisms publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.93.13.6371 contributor: fullname: Shemer – volume: 15 start-page: 290 year: 2003 ident: 10.1016/j.ygeno.2008.10.002_bib20 article-title: Reprogramming DNA methylation in the preimplantation stage: peeping with Dolly's eyes publication-title: Curr. Opin. Cell Biol. doi: 10.1016/S0955-0674(03)00031-0 contributor: fullname: Kang – volume: 88 start-page: 222 year: 2006 ident: 10.1016/j.ygeno.2008.10.002_bib15 article-title: The bovine IGF2 gene is differentially methylated in oocyte and sperm DNA publication-title: Genomics doi: 10.1016/j.ygeno.2006.03.011 contributor: fullname: Gebert – volume: 1518 start-page: 137 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib28 article-title: Epigenetic mark sequence of the H19 gene in human sperm publication-title: Biochim. Biophys. Acta. doi: 10.1016/S0167-4781(01)00190-7 contributor: fullname: Hamatani – volume: 293 start-page: 1086 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib3 article-title: Imprinting and the epigenetic asymmetry between parental genomes publication-title: Science doi: 10.1126/science.1064020 contributor: fullname: Ferguson-Smith – volume: 74 start-page: 574 year: 2007 ident: 10.1016/j.ygeno.2008.10.002_bib34 article-title: Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos publication-title: Mol. Reprod. Dev. doi: 10.1002/mrd.20620 contributor: fullname: Isom – volume: 26 start-page: 1245 year: 2006 ident: 10.1016/j.ygeno.2008.10.002_bib9 article-title: Developmental profile of H19 differentially methylated domain (DMD) deletion alleles reveals multiple roles of the DMD in regulating allelic expression and DNA methylation at the imprinted H19/Igf2 locus publication-title: Mol. Cell Biol. doi: 10.1128/MCB.26.4.1245-1258.2006 contributor: fullname: Thorvaldsen – volume: 12 start-page: 2873 year: 2003 ident: 10.1016/j.ygeno.2008.10.002_bib14 article-title: Methylation imprints of the imprint control region of the SNRPN-gene in human gametes and preimplantation embryos publication-title: Hum. Mol. Genet. doi: 10.1093/hmg/ddg315 contributor: fullname: Geuns – volume: 91 start-page: 121 year: 2008 ident: 10.1016/j.ygeno.2008.10.002_bib39 article-title: Diploid parthenogenetic embryos adopt a maternal-type methylation pattern on both sets of maternal chromosomes publication-title: Genomics doi: 10.1016/j.ygeno.2007.10.005 contributor: fullname: Liu – volume: 22 start-page: 2990 year: 1994 ident: 10.1016/j.ygeno.2008.10.002_bib45 article-title: High sensitivity mapping of methylated cytosines publication-title: Nucleic Acids Res. doi: 10.1093/nar/22.15.2990 contributor: fullname: Clark – volume: 6 start-page: 59 year: 1989 ident: 10.1016/j.ygeno.2008.10.002_bib1 article-title: Genomic imprinting in the mouse publication-title: Dev. Biol. contributor: fullname: Howlett – volume: 11 start-page: 631 year: 2005 ident: 10.1016/j.ygeno.2008.10.002_bib18 article-title: IVF results in de novo DNA methylation and histone methylation at an Igf2-H19 imprinting epigenetic switch publication-title: Mol. Hum. Reprod. doi: 10.1093/molehr/gah230 contributor: fullname: Li – volume: 84 start-page: 952 year: 2004 ident: 10.1016/j.ygeno.2008.10.002_bib21 article-title: Timing of establishment of paternal methylation imprints in the mouse publication-title: Genomics doi: 10.1016/j.ygeno.2004.08.012 contributor: fullname: Li – volume: 62 start-page: 1526 year: 2000 ident: 10.1016/j.ygeno.2008.10.002_bib40 article-title: Differential effects of culture on imprinted H19 expression in the preimplantation mouse embryo publication-title: Biol. Reprod. doi: 10.1095/biolreprod62.6.1526 contributor: fullname: Doherty – volume: 113 start-page: 6 year: 2006 ident: 10.1016/j.ygeno.2008.10.002_bib4 article-title: The continuing quest to comprehend genomic imprinting publication-title: Cytogenet. Genome Res. doi: 10.1159/000090808 contributor: fullname: Miyoshi – volume: 14 start-page: 1908 year: 2000 ident: 10.1016/j.ygeno.2008.10.002_bib6 article-title: A transcriptional insulator at the imprinted H19/Igf2 locus publication-title: Genes Dev. doi: 10.1101/gad.14.15.1908 contributor: fullname: Kaffer – volume: 10 start-page: 1711 year: 2000 ident: 10.1016/j.ygeno.2008.10.002_bib23 article-title: Novel imprinted DLK1/GTL2 domain on human chromosome 14 contains motifs that mimic those implicated in IGF2/H19 regulation publication-title: Genome Res. doi: 10.1101/gr.161600 contributor: fullname: Wylie – volume: 17 start-page: 4322 year: 1997 ident: 10.1016/j.ygeno.2008.10.002_bib8 article-title: A 5′ 2-kilobase-pair region of the imprinted mouse H19 gene exhibits exclusive paternal methylation throughout development publication-title: Mol. Cell Biol. doi: 10.1128/MCB.17.8.4322 contributor: fullname: Tremblay – volume: 293 start-page: 1089 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib30 article-title: Epigenetic reprogramming in mammalian development publication-title: Science doi: 10.1126/science.1063443 contributor: fullname: Reik – volume: 7 start-page: 419 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib42 article-title: Culture of preimplantation embryos and its long-term effects on gene expression and phenotype publication-title: Hum. Reprod. Update doi: 10.1093/humupd/7.4.419 contributor: fullname: Khosla – volume: 27 start-page: 101 year: 2002 ident: 10.1016/j.ygeno.2008.10.002_bib26 article-title: Identification and resolution of artifacts in bisulfite sequencing publication-title: Methods doi: 10.1016/S1046-2023(02)00060-9 contributor: fullname: Warnecke – volume: 10 start-page: 475 year: 2000 ident: 10.1016/j.ygeno.2008.10.002_bib31 article-title: Active demethylation of the paternal genome in the mouse zygote publication-title: Curr. Biol. doi: 10.1016/S0960-9822(00)00448-6 contributor: fullname: Oswald – volume: 87 start-page: 417 year: 2006 ident: 10.1016/j.ygeno.2008.10.002_bib12 article-title: Epigenetic status of the H19 locus in human oocytes following in vitro maturation publication-title: Genomics doi: 10.1016/j.ygeno.2005.10.008 contributor: fullname: Borghol – volume: 75 start-page: 81 year: 2005 ident: 10.1016/j.ygeno.2008.10.002_bib16 article-title: Epigenetic deregulation of genomic imprinting in human disorders and following assisted reproduction publication-title: Birth Defects Res. C Embryo Today doi: 10.1002/bdrc.20039 contributor: fullname: Arnaud – volume: 428 start-page: 860 year: 2004 ident: 10.1016/j.ygeno.2008.10.002_bib2 article-title: Birth of parthenogenetic mice that can develop to adulthood publication-title: Nature doi: 10.1038/nature02402 contributor: fullname: Kono – volume: 98 start-page: 13734 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib36 article-title: Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.241522698 contributor: fullname: Dean – volume: 12 start-page: 3669 year: 1993 ident: 10.1016/j.ygeno.2008.10.002_bib38 article-title: The ontogeny of allele-specific methylation associated with imprinted genes in the mouse publication-title: EMBO J. doi: 10.1002/j.1460-2075.1993.tb06041.x contributor: fullname: Brandeis – volume: 98 start-page: 72 year: 2007 ident: 10.1016/j.ygeno.2008.10.002_bib29 article-title: A comparative analysis of metabolism and viability in porcine oocytes during in vitro maturation publication-title: Anim. Reprod. Sci. doi: 10.1016/j.anireprosci.2006.10.006 contributor: fullname: Krisher – volume: 29 start-page: E65 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib25 article-title: Bisulfite genomic sequencing: systematic investigation of critical experimental parameters publication-title: Nucleic Acids Res. doi: 10.1093/nar/29.13.e65 contributor: fullname: Grunau – volume: 89 start-page: 1827 year: 1992 ident: 10.1016/j.ygeno.2008.10.002_bib24 article-title: A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.89.5.1827 contributor: fullname: Frommer – volume: 10 start-page: 2983 year: 2001 ident: 10.1016/j.ygeno.2008.10.002_bib33 article-title: Genome-wide methylation patterns in normal and uniparental early mouse embryos publication-title: Hum. Mol. Genet. doi: 10.1093/hmg/10.26.2983 contributor: fullname: Barton – volume: 66 start-page: 635 year: 2002 ident: 10.1016/j.ygeno.2008.10.002_bib35 article-title: Improvement of an electrical activation protocol for porcine oocytes publication-title: Biol. Reprod. doi: 10.1095/biolreprod66.3.635 contributor: fullname: Zhu – volume: 13 start-page: 388 year: 2002 ident: 10.1016/j.ygeno.2008.10.002_bib22 article-title: Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs publication-title: Mamm. Genome doi: 10.1007/s00335-001-3059-x contributor: fullname: Amarger – volume: 15 start-page: 2945 year: 2006 ident: 10.1016/j.ygeno.2008.10.002_bib27 article-title: CTCF binding sites promote transcription initiation and prevent DNA methylation on the maternal allele at the imprinted H19/Igf2 locus publication-title: Hum. Mol. Genet. doi: 10.1093/hmg/ddl237 contributor: fullname: Engel – volume: 51 start-page: 182 year: 1998 ident: 10.1016/j.ygeno.2008.10.002_bib11 article-title: Bisulfite sequencing in preimplantation embryos: DNA methylation profile of the upstream region of the mouse imprinted H19 gene publication-title: Genomics doi: 10.1006/geno.1998.5371 contributor: fullname: Warnecke – volume: 126 start-page: 213 year: 2006 ident: 10.1016/j.ygeno.2008.10.002_bib37 article-title: DNA methylation pattern in pig in vivo produced embryos publication-title: Histochem. Cell Biol. doi: 10.1007/s00418-006-0153-x contributor: fullname: Fulka – volume: 74 start-page: 307 year: 2006 ident: 10.1016/j.ygeno.2008.10.002_bib41 article-title: Effect of zona pellucida removal on DNA methylation in early mouse embryos publication-title: Biol. Reprod. doi: 10.1095/biolreprod.105.046284 contributor: fullname: Ribas – volume: 90 start-page: 1038 year: 2003 ident: 10.1016/j.ygeno.2008.10.002_bib7 article-title: Epigenetic regulation of Igf2/H19 imprinting at CTCF insulator binding sites publication-title: J. Cell Biochem. doi: 10.1002/jcb.10684 contributor: fullname: Yang – volume: 79 start-page: 530 year: 2002 ident: 10.1016/j.ygeno.2008.10.002_bib13 article-title: Methylation dynamics of imprinted genes in mouse germ cells publication-title: Genomics doi: 10.1006/geno.2002.6732 contributor: fullname: Lucifero – volume: 69 start-page: 902 year: 2003 ident: 10.1016/j.ygeno.2008.10.002_bib17 article-title: Disruption of imprinted gene methylation and expression in cloned preimplantation stage mouse embryos publication-title: Biol. Reprod. doi: 10.1095/biolreprod.103.017293 contributor: fullname: Mann – volume: 17 start-page: 275 year: 1997 ident: 10.1016/j.ygeno.2008.10.002_bib10 article-title: The pre-implantation ontogeny of the H19 methylation imprint publication-title: Nat. Genet. doi: 10.1038/ng1197-275 contributor: fullname: Olek – volume: 362 start-page: 751 year: 1993 ident: 10.1016/j.ygeno.2008.10.002_bib5 article-title: Parental-origin-specific epigenetic modification of the mouse H19 gene publication-title: Nature doi: 10.1038/362751a0 contributor: fullname: Ferguson-Smith – volume: 34 start-page: 43 year: 1993 ident: 10.1016/j.ygeno.2008.10.002_bib44 article-title: Development of androgenetic mouse embryos produced by in vitro fertilization of enucleated oocytes publication-title: Mol. Reprod. Dev. doi: 10.1002/mrd.1080340107 contributor: fullname: Kono |
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| SubjectTerms | Androgenesis Animals Biological and medical sciences Blastocyst - metabolism Cell differentiation, maturation, development, hematopoiesis Cell physiology DNA Methylation Female Fundamental and applied biological sciences. Psychology Genes. Genome Genetics of eukaryotes. Biological and molecular evolution Genomic Imprinting Germ Cells - metabolism Imprinting DNA methylation Insulin-Like Growth Factor II - genetics Insulin-Like Growth Factor II - metabolism Molecular and cellular biology Molecular genetics Parthenogenesis Pregnancy Preimplantation Sus scrofa |
| Title | Methylation status of differentially methylated regions at Igf2/H19 locus in porcine gametes and preimplantation embryos |
| URI | https://dx.doi.org/10.1016/j.ygeno.2008.10.002 https://www.ncbi.nlm.nih.gov/pubmed/18983907 https://search.proquest.com/docview/20277325 https://search.proquest.com/docview/66838835 |
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