The Tight Junction Protein ZO-1 Is Dispensable for Barrier Function but Critical for Effective Mucosal Repair

• Published in: Gastroenterology (New York, N.Y. 1943) Vol. 161; no. 6; pp. 1924 - 1939
• Main Authors: Kuo, Wei-Ting, Zuo, Li, Odenwald, Matthew A., Madha, Shariq, Singh, Gurminder, Gurniak, Christine B., Abraham, Clara, Turner, Jerrold R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2021
• Subjects: Animals; Cell Proliferation; Cells, Cultured; Colon - metabolism; Colon - pathology; Databases, Genetic; Disease Models, Animal; Epithelial Cells - metabolism; Epithelial Cells - pathology; Humans; Inflammatory Bowel Disease; Inflammatory Bowel Diseases - genetics; Inflammatory Bowel Diseases - metabolism; Inflammatory Bowel Diseases - pathology; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Intestinal Permeability; Mice; Mice, Knockout; Mitosis; Mitotic Spindle; Permeability; Spindle Apparatus - genetics; Spindle Apparatus - metabolism; Spindle Apparatus - pathology; Wnt; Wnt Signaling Pathway; Wound Healing; Zonula Occludens-1 Protein - genetics; Zonula Occludens-1 Protein - metabolism; Mitosis; DSS; qRT-PCR; GSEA; Mitotic Spindle; IBD; mRNA; TNBS; 4-OHT; ZO-1; EdU; Intestinal Permeability; Inflammatory Bowel Disease; MDCK; Wnt; WT; NuMA

Increased permeability is implicated in the pathogenesis of intestinal disease. In vitro and in vivo studies have linked down-regulation of the scaffolding protein ZO-1, encoded by the TJP1 gene, to increased tight junction permeability. This has not, however, been tested in vivo. Here, we assessed...