A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone

• Published in: Nature protocols Vol. 5; no. 3; pp. 550 - 560
• Main Authors: Zhu, Heng, Guo, Zi-Kuan, Jiang, Xiao-Xia, Li, Hong, Wang, Xiao-Yan, Yao, Hui-Yu, Zhang, Yi, Mao, Ning
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2010; Nature Publishing Group
• Subjects: 631/1647/1407/651; 631/1647/2230; 631/1647/334/1874/345; 631/532/2074; Analytical Chemistry; Animals; Biological Techniques; Biomedical and Life Sciences; Bone marrow; Bone Marrow Cells - cytology; Cell Adhesion; Cell Culture Techniques - methods; Cell Differentiation; Cell Separation - methods; Cells, Cultured; Collagenases; Compact bone; Computational Biology/Bioinformatics; Female; Flushing; Hematopoietic Stem Cells - cytology; Identification and classification; Life Sciences; Male; Mesenchymal Stromal Cells - cytology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Microarrays; Multipotent Stem Cells - cytology; Organic Chemistry; Physiological aspects; protocol; Stem cells; China

Unlike humans, mouse bone marrow-derived mesenchymal stem cells (MSCs) cannot be easily harvested by adherence to plastic owing to the contamination of cultures by hematopoietic cells. The design of the protocol described here is based on the phenomenon that compact bones abound in MSCs and hematopo...